摘要
目的:探讨丹皮酚对动脉粥样硬化(AS)大鼠脂质代谢和核因子-κB(NF-κB)的影响。方法:采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、辛伐他汀组(10 mg·kg-1)、丹皮酚高剂量组和低剂量组(20,10 mg·kg-1),每组8只。灌胃给药4周后,各组取血清,测定血脂水平;取主动脉切片,光镜下作病变分级评分,免疫组化法检测NF-κB的表达。结果:丹皮酚高剂量组能改善AS大鼠的主动脉病变,与模型组比较有统计学意义(P<0.05);丹皮酚高、低剂量组可明显降低AS大鼠血清三酰甘油(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平(P<0.05或P<0.01);丹皮酚可下调主动脉NF-κB表达,高剂量组IOD值(1.96±0.55)和低剂量组IOD值(2.50±0.80)与模型组(3.65±1.14)比较,差异均有统计学意义(P<0.05或0.01)。结论:丹皮酚治疗大鼠AS的作用机制与调节脂质代谢、下调主动脉NF-κB的表达有关。
Objective： To investigate the effects of paeonol on aortic nuclear factor-kappaB（ NF-κB） activity and blood lipid levels in the rats with atherosclerosis（ AS）. Methods： The AS rat model was established by administration of high-fat diet and vitamin D3（ i.p.）. The rats were divided into 5 groups（ n = 8） randomly,namely the normal group,model group,positive control group（ simvastatin,10 mg·kg- 1） and paeonol groups with the dose of 20 and 10 mg·kg- 1,respectively. After the 4-week treatment,the serum of rats was collected to determine the lipid levels. The aortic pathologic changes of AS rats were observed under a microscope,and then graded,and aortic NF-κB activity was detected by an immunohistochemistry method. Results： Administration of paeonol improved the severity of aortic pathology in AS rats. The pathologic grade in the paeonol group（ 20 mg·kg- 1） showed a significant difference compared with that in the model group（ P〈0. 05）. Paeonol（ 20 and 10 mg·kg- 1） markedly decreased the TG,TC and LDL-C levels in the serum of AS rats（ P〈0. 05 or P〈0. 01）. Meanwhile,paeonol significantly inhibited the aortic NF-κB activity（ IOD value） with（ 1.96 ±0.55） and（ 2.50 ±0.80） in 20 and 10 mg·kg- 1group,respectively,which showed significant differences compared with that in the model group（ P〈0. 05 or P〈 0. 01）. Conclusion： Paeonol shows certain therapeutic effect in AS rats,and the mechanisms may be related with the regulation of lipid mobilization and the inhibition of aortic NF-κB activity.
出处
《中国药师》
CAS
2014年第9期1441-1443,共3页
China Pharmacist
基金
国家自然科学基金项目(编号:81072969)
