摘要
目的探讨甲基化敏感性高分辨率熔解曲线分析(MS-HRM)在遗传性非息肉性大肠癌(HNPCC)筛查中的应用价值。方法采用实时荧光定量PCR技术检测miR-195在41例散发性大肠癌(SCRC)和9例HNPCC患者癌组织及对应癌旁正常组织(距离癌组织>5 cm)中的表达水平,随后使用MS-HRM检测miR-195启动子区域CpG岛甲基化情况。结果 miR-195在HNPCC癌组织中的表达量为1.20±1.48,甲基化比例为55.56%(5/9);miR-195在SCRC组织中的表达量为0.76±1.06,甲基化比例为58.54%(24/41),差异无统计学意义(P>0.05);miR-195在肠癌组织及癌旁正常组织中的平均表达水平分别为0.837±1.145和2.236±2.468,差异具有统计学意义(P<0.05)。结论 miR-195在SCRC和HNPCC癌组织中的表达有无差异尚待进一步研究。miR-195可能有助于抑制肠癌发展,并且因其甲基化而参于大肠癌的发病机制。
Objective To investigate the application value of methylation-specific high-resolution melting analysis （MS-HRM） for screening hereditary non-polyposis colorectal cancer （HNPCC）.Methods The expression of miR-195 in 41cases of SCRC and 9 cases of HNPCC and adjacent non-tumor mucosa tissues （5 cm away from the cancer tissue）were detected by Real-time PCR.Then MS-HRM was used to detect the methylation of miR-195 promoter regions CpG island.Results The expression of miR-195 in HNPCC and SCRC cancer tissue were 1.20 ± 1.48,0.76 ± 1.06,respectively.MS-HRM showed that the methylation rates of miR-195 in HNPCC cancer tissue and SCRC cancer tiscues were 55.56％ （5/9）,58.54％ （24/41）,respectively.There was no statistically significant difference （P ＞ 0.05）.However,the average expression of miR-195 in human colon carcinoma and adjacent normal mucosa were 0.837 ± 1.145 and 2.236 ± 2.468,there was significant difference （P ＜ 0.05）.Conclusion The differential expression of miR-195 between HNPCC and SCRC needs further research.miR-19 may inhibit the development of colorectal cancer,and participate in the pathogenesis of colorectal cancer for methylation.
出处
《胃肠病学和肝病学杂志》
CAS
2014年第9期1047-1049,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
广东省自然科学基金资助项目(No:S2011010005494)
广东省惠州市科技计划项目(No:2011Y091)
