摘要
以中间偃麦草(Thinopyrum intermedium,2n=42)与普通小麦‘烟农15’杂交,从其杂种后代中选育出一个细胞学稳定的二体异附加系‘山农120211’,该研究对其细胞学和主要性状特点进行了鉴定。白粉病抗性鉴定结果表明,‘山农120211’成株期对白粉病的田间抗性为免疫,苗期对白粉病菌种E09表现为免疫。以耐盐品种‘山融3号’为对照进行苗期耐盐性鉴定表明,‘山农120211’耐盐级别为2级(较强)。细胞学鉴定表明:‘山农120211’根尖细胞染色体数目为2n=44,PMC MI染色体构型为2n=22Ⅱ,具有高度的细胞学稳定性。以拟鹅观草基因组DNA为探针,‘烟农15’DNA为封阻,在‘山农120211’的根尖有丝分裂细胞中检测到2条染色体具有明显的杂交信号,确定其为二体异附加系。利用该实验室筛选的71对E组染色体特异分子标记,对‘山农120211’分析显示,标记BE494262在中间偃麦草和‘山农120211’中可以稳定扩增出1条440bp特异带,而‘烟农15’中缺少此带,BE494262可作为‘山农120211’中附加中间偃麦草染色体的特异标记。利用二倍体长穗偃麦草和一套中国春-长穗偃麦草异附加系(1Ee^7Ee),进一步将BE494262定位在2Ee染色体,确定‘山农120211’所附加的中间偃麦草染色体为2Ee染色体。
An alien disomic addition line,‘ Shannong 120211’,which derived from the progeny of Thinopyrum intermedium and common wheat cv.‘Yannong15’,were identified in morphology,cytology,genome in situ hybridization（GISH）,powdery mildew resistance and salt tolerance.Resistance identification of adult in field and seeding in lab showed that ‘Shannong 120211’ was nearly immune to powdery mildew.Compared with the‘Shanrong 3’,which was highly resistant to salt,the result of salt tolerance is level 2.The results demonstrated that the main morphological traits of ‘Shannong 120211’,with the chromosome num ber of 2n=44 in root tip cells and chromosome configuration of 2n=22 Ⅱ at PMC MI.The results of GISH showed that there were two chromosomes of ‘Shannong 120211’ with obvious hybridization signals,which further proved that ‘Shannong 120211’ was an alien disomic addition line with a pair of Th.intermedium chromosomes in wheat background.Using 71 pairs chromosome specific molecular markers of group E to analyze the ‘Shannong120211’,we found marker BE494262 could reveal the specific band of Th.intermedium and be used to identify ‘Shannong 120211’.Using Thinopyrum elongatum and the addition line of Cs-Thinopyrum elongata （1Ee-TEe） to further located the BE494262 at 2Ee,which identified that the added chromosomes of ‘Shannong 120211’ came from the 2Ee of T.intermedium.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第9期1757-1763,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家高技术研究发展计划(2011AA100103)
关键词
小麦
中间偃麦草
抗白粉病
耐盐性
GISH
SSR
wheat
Thinopyrum intermedium
powdery mildew resistance
salt tolerance
GISH
SSR
