摘要
目的：通过生物信息学方法预测德国小蠊致敏原蛋白Bla g 8的三维结构和抗原表位,为蟑螂引起人类变态反应性疾病的诊断和治疗提供线索.方法：在美国国立生物信息中心（NCBI）上获取Bla g 8致敏原蛋白序列,通过序列比对（BLAST）得到相似序列,应用MEGA6.0构建同源进化树;联合应用DNAStar多种方法预测其抗原表位,使用Modeller 9.12同源建模后经Gromacs能量优化获取稳定的致敏原蛋白.结果：德国小蠊Bla g 8与美洲大蠊肌球蛋白轻链在进化上具有较近的亲缘关系;Bla g 8为含有2个EF手钙结合蛋白位点的α结构疏水性蛋白,主要抗原表位集中于1～24,27 ～ 50,55 ～ 65,69 ～91,92 ～110,121～132,138 ～ 153,159 ～ 180,191 ～208区域;预测并经能量优化后得到稳定的三维结构.结论：通过预测Bla g 8结构及抗原表位,为筛选低致敏原衍生物提供了分子基础.
Objective：To predict the structures and antigenic epitopes of allergen Bla g 8 of German cockroach with bioinfomatics methods,and so as to provide clues for diagnosis and treatment of human allergic diseases caused by cockroach.Methods：The sequences of Bla g 8 were obtained from USA national center for biotechnology information （NCBI）.Sequence alignment （BLAST） was applied to search for the similar protein of Bla g 8.MEGA6.0 was used for constructing phylogenetic tree.Antigenic epitopes of Bla g 8 were predicted by the software of DNAStar.Homologous modeling was carried out with the Modeler 9.12,and stable allergen was obtained through energy optimization with Gromacs.Results：Bla g 8 and myosin light chain of Periplaneta americana had a close genetic relationship in evolution.Bla g 8 had hydrophobic proteins of alpha secondary structure containing 2 EF hand calcium binding sites.Its antigenic epitope mostly located on 1 ～ 24,27 ～ 50,55 ～ 65,69 ～ 91,92 ～ 110,121 ～ 132,138 ～ 153,159 ～ 180 and 191 ～208 regions.Stable three dimensional structure was obtained through energy optimization.Conclusions：It provides molecular basis for screening hypoallergenic variant of cockroach allergens through predicting the structures and antigenic epitopes of Bla g 8.
出处
《贵阳医学院学报》
CAS
2014年第5期669-674,共6页
Journal of Guiyang Medical College
基金
贵州省攻关项目[黔科合S系(20071038)]
贵州省教育厅自然科学研究项目[黔教合KY字(2012)039]
贵阳医学院研究生创新计划专项基金
关键词
蠊
致敏原
BLA
g
8
抗原
表位
蛋白质序列分析
Blattella germanica
sensitinogen
Bla g 8
antigen
epitope
protein sequence analysis
