摘要
目的观察黄柏煎剂的抗炎、抗菌作用。方法昆明种小鼠50只按体质量随机分为5组,小鼠连续灌胃黄柏煎剂7天后小鼠左耳廓内外侧涂20μL二甲苯致炎,右耳不涂作为对照,计算各组耳肿胀度及抑制率;SPF级SD大鼠40只在左、右前肢分别埋入无菌塑料环致肉芽肿,然后按体质量随机分为5组并按照相应剂量连续灌胃黄柏煎剂7 d,计算各组肉芽肿质量;昆明种小鼠60只,根据性别和体质量随机分为6组,分别按照相应剂量连续灌胃黄柏煎剂7 d。第7天给药1 h后各组小鼠腹腔注射金黄色葡萄球菌菌液,6 h再给药1次。观察小鼠在72 h内的死亡率。结果与对照组比较,黄柏煎剂各剂量组均能不同程度的抑制小鼠耳廓肿胀度,差异具有统计学意义(P<0.05或P<0.01),其中以低剂量组的作用最好;与对照组比较,黄柏煎剂低剂量组能显著降低大鼠肉芽肿质量(P<0.05);高、中剂量组有一定的降低趋势,与对照组比较,差异无统计学意义(P>0.05);黄柏高、中、低剂量组的死亡率分别是50%、40%、20%。与对照组比较,中、低剂量组可降低感染小鼠的死亡率(P<0.05或P<0.01)。结论黄柏煎剂具有良好的抗炎、抗菌作用。
Objective To observe the anti-inflammatory and antibacteria effect of Cortex Phellodendri Chinensis decoction（CPCD）. Method 50 Kunming mice were randomly divided into 5 groups, The mice in the study groups were administered intragastrically with CPCD, the outer ears of all mice were inflamed in left as experimental group and the right ears were not treated as a control group after treated 7 days, and then, each ear of all mice were calculated the swelling and suppression mice ; 40 SPF SD rats in the left and right forelimbs were made granulomas, and then all rats were randomly divided into five groups based on the weight, and the rats were administered intragastrically with CPCD within 7 days, then, the weight of each group granulomas were calculated; 60 Kunming mice were randomly divided into six groups according to sex and body weight, all groups mice were administered intragastrically with CPCD within 7 days according to the corresponding dose, and then, each group were administered with intraperitoneal injection of Staphylococcus aureus bacteria in the first seven days of administered after 1 h, in the first seven days of administered 6 h the mice were administered once more, mortality of mice was observed in 72 hrs. Result Experimental groups were compared with control groups, each dose group which was administered intragastrically with CPCD could inhibit ears swelling of mice in different levels, the difference was statistically significant （P 〈 O. 05 or P 〈 0.01 ） , of which the best group was low-dose group; Experimental groups were compared with the control group, low-dose groups which was administered intragastrically with CPCD could significantly reduce the weights of rat granulomas （P 〈 0.05） , and high dose group and medium dose group could not significantly reduce the weights of rat granulomas which were compared with the control group, the differences were not statistically significant （P 〉 0.05 ） ; The mice were treats with high, medium and low-dose groups, the mortality of mice were 50% , 40% , 20% , which were compared with the control group, the low-dose group can reduce the mortality of the mice （P 〈 O. 05 or P 〈 0.01 ）. Conclusion CPCD had anti-inflammatory and antibacteria effect.
出处
《实验动物科学》
2014年第4期14-17,共4页
Laboratory Animal Science
关键词
黄柏煎剂
抗炎
抗菌
Cortex Phellodendri Chinensis decoction
anti-inflammatory
antibacteria
