摘要
目的:优选川芎和细辛挥发油的β-环糊精(β-CD)微囊萃取工艺条件。方法:选择β-CD和硫酸钠作为双水相萃取剂,以挥发油收率、甲基丁香酚和苯酞类物质(以藁本内酯计)质量的综合评分为指标,通过正交试验考察萃取液中β-环糊精和硫酸钠的质量浓度、提取方式、萃取温度对微囊萃取工艺的影响。采用GC-MS分析挥发油的组分,以邻二甲苯和邻苯二甲酸二丁酯为内标物。结果:最佳萃取工艺条件为加3倍量40%乙醇室温下浸泡24 h,60℃热浸60 min,抽滤,提取液中加入β-CD和硫酸钠至质量浓度分别为300,50 g·L-1,50℃振荡萃取30 min;挥发油收率86.54%,挥发油中甲基丁香酚和苯酞类物质(以藁本内酯计)质量分别为75.88,353.91 mg。结论:β-CD微囊双水相萃取及包合川芎和细辛挥发油的工艺合理可行,适合于采用β-CD包合的挥发油制剂工业化生产。
Objective：To optimize β-cyclodextrin （β-CD） microcapsule extraction process of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma.Method：β-CD and sodium sulfate were employed as extraction agents in aqueous two-phase system,with composite score of volatile oil yield,quality of methyl eugenol and phthalides （in the content of ligustilide） as index,L9 （3^4） orthogonal test was adopted to optimize extraction and inclusion technology of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by β-CD microcapsule,the concentration of β-CD and sodium sulfate in extract,extraction methods and temperature were selected as main influence factors.Taking O-xylene and dibutyl phthalate as internal standards,components of volatile oil was analyzed by GC-MS.Result：Optimal extraction process conditions were as follows：soaked 24 h with 3 times the amount of 40% ethanol,heated to temperature of 60 ℃ and stirred for 60 min,then vacuum suction filtrated,β-CD and sodium sulfate were added to filtrate to the concentration of 300 g · L^-1 and of 50 g·L^-1,respectively; stirred and extracted for 30 min at 50 ℃.Under these conditions,vield of volatile oil was 86.54%,contents of methyl eugenol and phthalides （in the content of ligustilide） were 75.88 mg and 353.91 mg.Conclusion：Technologies for extraction and inclusion of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma are feasible and suitable for industrial production of volatile oil preparations for3-CD inclusion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第20期1-5,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广州中医药大学邓铁涛基金项日(2005D020)
关键词
川芎
细辛
Β-环糊精
甲基丁香酚
微囊双水相萃取法
藁本内酯
内标物
Chuanxiong Rhizoma
Asari Radix et Rhizoma
β-cyclodextrin
methyl eugenol
microcapsule aqueous ewo-phase system
ligustilide
internal standard
