摘要
目的:观察贯叶连翘灌胃联合光动力疗法对类风湿关节炎白兔膝关节滑膜组织病理形态的影响。方法:将36只12周龄新西兰大白兔随机分为正常对照组(空白组)12只、模型组12只、治疗组12只。模型组和治疗组用鸡卵蛋白诱导建立类风湿关节炎模型。造模后1周,治疗组以1 500 mg·mL-1贯叶连翘药液灌胃,空白组和模型组以生理盐水灌胃,每天1次,连续灌胃7 d。随机选取治疗组大白兔一侧膝关节作为贯叶连翘联合光动力疗法组,另一侧膝关节作为贯叶连翘组。贯叶连翘联合光动力疗法组于每次灌胃后2 h采用氦氖激光治疗仪照射膝关节,每天1次,连续照射7 d;贯叶连翘组膝关节避光。治疗结束后2周,处死白兔,取各组白兔膝关节并分离出滑膜组织,采用HE染色法观察膝关节滑膜的组织形态学变化,并按照滑膜病理5级评分法对炎性细胞浸润、滑膜细胞增生及滑膜纤维组织增生3项指标进行评分。结果:1膝关节滑膜形态观察。空白组滑膜衬里层细胞多呈单层,排列规则,滑膜组织较为疏松,无炎性细胞浸润;贯叶连翘联合光动力疗法组滑膜细胞增生层数较少,炎性细胞浸润程度及滑膜纤维组织增生程度较模型组及贯叶连翘组明显减轻。2膝关节滑膜病理指标评分。各组滑膜炎性细胞浸润评分比较,差异有统计学意义(F=30.130,P=0.000)。组间两两比较,空白组评分[(0.50±0.36)分]低于模型组[(2.92±1.00)分]、贯叶连翘组[(2.67±0.65)分]和贯叶连翘联合光动力疗法组[(1.58±0.51)分][q=11.973,P=0.000;q=10.735,P=0.000;q=5.365,P=0.000];模型组评分高于贯叶连翘联合光动力疗法组(q=6.603,P=0.000);模型组评分与贯叶连翘组比较,差异无统计学意义(q=1.238,P=0.386);贯叶连翘联合光动力疗法组评分低于贯叶连翘组(q=5.365,P=0.000)。各组滑膜细胞增生评分比较,差异有统计学意义(F=30.910,P=0.000)。组间两两比较,空白组评分[(0.92±0.67)分]低于模型组[(3.33±0.78)分]、贯叶连翘组[(2.92±0.67)分]和贯叶连翘联合光动力疗法组[(1.75±0.62)分](q=12.187,P=0.000;q=10.084,P=0.000;q=4.200,P=0.005);模型组评分高于贯叶连翘联合光动力疗法组(q=7.982,P=0.000);模型组评分与贯叶连翘组比较,差异无统计学意义(q=2.103,P=0.144);贯叶连翘联合光动力疗法组评分低于贯叶连翘组(q=5.884,P=0.000)。各组滑膜纤维组织增生评分比较,差异有统计学意义(F=25.700,P=0.000)。组间两两比较,空白组评分[(0.75±0.62)分]低于模型组[(2.83±0.83)分]、贯叶连翘组[(2.67±0.49)分]和贯叶连翘联合光动力疗法组[(1.67±0.65)分](q=10.903,P=0.000;q=10.034,P=0.000;q=4.780,P=0.001);模型组评分高于贯叶连翘联合光动力疗法组(q=6.108,P=0.000);模型组评分与贯叶连翘组比较,差异无统计学意义(q=0.874,P=0.540);贯叶连翘联合光动力疗法组评分低于贯叶连翘组(q=5.234,P=0.001)。结论:贯叶连翘灌胃联合光动力疗法能有效地抑制类风湿关节炎白兔膝关节炎性滑膜细胞浸润,减轻其滑膜细胞增生和滑膜纤维组织增生,但其具体作用机制尚不明确,有待进一步研究。
Objective：To study the effect of intragastric administration of hypericum perforatum combined with photodynamic therapy on the pathological morphology of synovial tissue of knee joints in rabbits with rheumatoid arthritis.Methods：Thirty-six 1 2-week-old New Zealand white rabbits were randomly divided into 3 groups,1 2 cases in normal control group（blank group）,1 2 cases in model group,and 1 2 cases in treatment group.Rabbits in model group and treatment group were induced into the models of rheumatoid arthritis by using chicken ovalbumin.Rabbits in treatment group were intragastric administrated with the liquor of hyperforin perforatum with dose of 1 500 mg/mL,while rabbits in blank group and model group were intragastric administrated with normal saline 1 week after modeling.Rabbits in each group were intragastric administrated once a day for consecutive 7 days.Unilateral knee joints of these rabbits in treatment group were ran-domly recruited into hyperforin perforatum combined with photodynamic therapy group（combination therapy group）,and the others were re-cruited into hyperforin perforatum group.The knee joints of former group were treated with He-Ne laser irradiation 2 hours after intragastric administration once a day for consecutive 7 days,while the knee joints of latter group were protected from light.These rabbits were executed and the knee joints were fetched out for separating synovial tissues 2 weeks after the end of treatment.The morphological changes of synovial tissues in knee joints were observed after HE staining;and inflammatory cell infiltration,synovial cell hyperplasia and synovial fibroplasia were assessed according to the synovial pathological scores.Results：Synovial lining cells spread in monolayer regularly in blank group,and the synovial tissue was relative sparsate and no inflammatory cells infiltrating were found.Less layer of synovial cells hyperplasia was found in combination therapy group,and the degree of inflammatory cell infiltration and synovial fibroplasia were alleviated significantly,compared to model group and hyperforin perforatum group.There were statistical differences in the scores of synovial inflammatory cell infiltration be-tween the groups（F=30.1 30,P=0.000）.Further pairwise comparison showed that the scores of blank group（0.50＋/-0.36 points）were lower than those of model group（2.92＋/-1 .00 points）,hyperforin perforatum group（2.67＋/-0.65 points）and combination therapy group （1 .58＋/-0.51 points）（q=1 1 .973,P=0.000;q=1 0.735,P=0.000;q=5.365,P=0.000）;Model group surpassed combination ther-apy group in scores（q=6.603,P=0.000）;There were no statistical differences in the scores between model group and hyperforin perfora-tum group（q=1 .238,P=0.386）;The scores of combination therapy group were lower than those of hyperforin perforatum group（q=5.365,P=0.000）.There were statistical differences in the scores of synovial cell hyperplasia between the groups（F=30.910,P=0.000）.Further pairwise comparison showed that the scores of blank group（0.92 ＋/-0.67 points）were lower than those of model group （3.33＋/-0.78 points）,hyperforin perforatum group（2.92＋/-0.67 points）and combination therapy group（1 .75 ＋/-0.62 points）（q=12.187,P=0.000;q =10.084,P=0.000;q =4.200,P=0.005）.Model group surpassed combination therapy group in scores（q =7.982,P=0.000）;There were no statistical differences in the scores between model group and hyperforin perforatum group（q=2.1 03, P=0.1 44）;The scores of combination therapy group were lower than those of hyperforin perforatum group（q=5.884,P=0.000）.There were statistical differences in the scores of synovial fibroplasia between the groups（F=25.700,P=0.000）.Further pairwise comparison showed that the scores of blank group（0.75 ＋/-0.62 points）were lower than those of model group（2.83＋/-0.83 points）,hyperforin per-foratum group（2.67＋/-0.49 points）,and combination therapy group（1 .67 ＋/-0.65 points）（q=1 0.903,P=0.000;q=1 0.034,P=0.000;q=4.780,P=0.001 ）.Model group surpassed combination therapy group（q=6.1 08,P=0.000）;There were no statistical differ-ences in the scores between model group and hyperforin perforatum group（q=0.874,P=0.540）.The scores of combination therapy group were lower than those of hyperforin perforatum group（q=5.234,P=0.001 ）.Conclusion：Intragastric administration of hyperforin perfora-tum combined with photodynamic therapy can effectively inhibit inflammatory cell infiltration and reduce synovial cell hyperplasia and synovi-al fibroplasia in knee joints of rabbits with rheumatoid arthritis,but its specific mechanism of action is unclear and need to be further studied.
出处
《中医正骨》
2014年第9期6-10,共5页
The Journal of Traditional Chinese Orthopedics and Traumatology
关键词
关节炎
类风湿
光化学疗法
贯叶连翘
金丝桃素
病理形态学
兔
动物实验
Arthritis,rheumatoid
Photochemotherapy
Hypericum perforatum
Hypericin
Pathomorphism
Rabbits
Animal experimentation
