摘要
目的比较女贞子生品及酒蒸炮制品对肾阴虚模型大鼠睾丸组织氧化应激损伤的保护作用。方法 SD大鼠灌服甲状腺素片诱导肾阴虚模型,然后分别给予女贞子生品及酒蒸4 h、酒蒸8 h炮制品提取液进行干预,空白对照组给予等体积的蒸馏水,各组连续灌胃24 d。用黄嘌呤氧化酶法测定大鼠睾丸组织中SOD活力,用硫代巴比妥酸法测定大鼠睾丸组织中MDA水平,用重量法测定大鼠睾丸及免疫器官指数。结果与空白对照组比较,肾阴虚模型大鼠睾丸组织中SOD活力显著降低,MDA水平显著升高,睾丸、胸腺、肝脏、肾脏及脾脏指数明显下降。女贞子生品及酒蒸品均能显著提高肾阴虚模型大鼠的睾丸、胸腺、肝脏、脾脏及肾脏的脏器指数,增强睾丸组织SOD和降低MDA水平,但均以酒蒸8 h炮制品作用最佳。结论女贞子酒蒸后可增强对肾阴虚模型大鼠睾丸组织氧化应激损伤的保护作用。
AIM To compare the protective effects on oxidative damag ciency between Ligustri lucidi Fructus and steamed with wine. METHODS e to testis of rats with kidney-yin deft- Experimental SD rats model for kid- ney-yin deficiency were made by garage receiving thyroxine tablets. Then these rats were fed on extract of steamed with wine for 4 h, 8 h, and raw L. htcidi Fructus, respectively, for 24 days and the control group were given dis-tilled water at the same dose. The activity of SOD in testis of rats was determined by xanthine oxidase assay. The MDA contents in testis of rats were measured by the method of thiobarbituric acid reaction. The testis and immune organ index of rats were determined by gravimetric method. RESULTS Compared with the blank control group, SOD activity significantly decreased and MDA content increased in testis of rats with kidney-yin deficiency. At the same time , testis, thymus, liver, kidney and spleen index decreased significantly. All extracts of L. lucidi Fruc- tus could increase the organ index , especially the samples steamed with wine for 8 h. The two samples steamed with wine samples could increase SOD activity and decrease MDA content in testis of rats with kidney-yin deficien- cy. The samples steamed with wine for 8 hours was better. CONCLUSION Ligustri lucidi Fructus steamed with wine can enhance the protective effects on the oxidative stress damage to testis of rats with kidney-yin deficiency.
出处
《中成药》
CAS
CSCD
北大核心
2014年第10期2144-2147,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81373968)
关键词
女贞子炮制品
肾阴虚大鼠
睾丸组织
SOD
MDA
steaming with wine
Ligustri lucidi Fructus
rats with kidney-yin deficiency
testis
SOD
MDA
