摘要
目的建立LC/MS/MS法同时检测动物组织中11种β2兴奋剂的方法。方法食品样品添加内标溶液后经酶水解、乙酸钠溶液提取,MCX阳离子交换柱净化,在MRM模式下用LC/MS/MS测定动物组织中11种β2兴奋剂的含量。结果 11种β2兴奋剂在猪肉中的加标回收率为80%~107%,相对标准偏差为5.5%~11.8%;在0.5μg/L^200μg/L浓度范围内相关系数>0.9961;方法检出限<0.05μg/kg、定量限<0.2μg/kg。结论该法具有简单、快速、灵敏等优点,并可同时对动物性食品中多种β2受体激动剂进行定性确证和定量测定,回收率达到较好水平,适合对动物性食品中β2受体激动剂残留的分析检测。
Objective To establish a method for simultaneous detection of 11 β2- agonists in animal tissues by LC- MS /MS.Methods The animal tissues were enzymatic hydrolyzed,extracted with sodium acetate solution and finally purified with a MCX cation- exchange column. The quantification was determination by LC- MS /MS in multiple reaction monitoring（ MRM）mode using isotope dilution internal standard method. Results The recoveries for 11 β2- agonists in pork samples were 80%to 107%,and the relative standard deviations（ RSDs） ranged was 5. 5% to 11. 8%. The linearities of the method were in the range of 0. 5 μg /L ~ 200 μg /L,with the correlation coefficients（ r） of greater than 0. 9961. The limits of detection were less than 0. 05 μg /kg and the limits of quantification were less than 0. 2 μg /kg. Conclusion This method is simple,rapid,and sensitive. It achieves the detection and confirmation of various β2- agonists in animal tissues simultaneously with a respectively good recovery level. Therefore,this method is suitable for determination of β2- agonist residues in animal tissues.
出处
《中国卫生检验杂志》
北大核心
2014年第18期2585-2588,共4页
Chinese Journal of Health Laboratory Technology
基金
厦门市科技计划指导性项目资助(3502z20089027)
关键词
三重四级杆液质联用仪
动物组织
β2-兴奋剂
氘代同位素
Triple quadrupole liquid chromatography - tandem mass spectrometry
Animal tissue
β2 - Agonists
Deuterium isotope
