摘要
目的观察大隐静脉和脾静脉滋养血管内皮细胞超微结构变化,探讨高流体静力压和缺氧对滋养血管内皮细胞的影响。方法收集曲张大隐静脉和高压性脾静脉管壁标本34例,另设对照组为正常大隐静脉和脾静脉34例。采用HE染色光镜下观察大隐静脉和脾静脉外膜层滋养血管,半薄切片定位。再采用超薄切片,透射电镜下观察滋养血管内皮细胞的超微结构变化。结果大隐静脉曲张组和病脾静脉组滋养血管内皮细胞细胞核结构完整,染色质分布正常;部分线粒体基质深染,嵴模糊、嵴断裂。结论高流体静力压和缺氧下大隐静脉和脾静脉管壁滋养血管内皮细胞的超微结构会出现重塑现象,二者改变相同。
Objectivev To observe the ultrastructural changes of vasa vasorum endothelial cells in the walls of the great saphenous vein and splenic vein, and to evaluate the effect of high hydrostatic pressure and hypoxia upon vasa vasorum endothelial cells. Methods Thirty-four varicose great saphenous vein samples and splenic vein samples with portal hypertension were obtained, and the same number of normal great saphenous vein and splenic vein were used as the control groups. Semi-thin sections stained with HE staining vasa vasorum of the adventitia in great saphenous vein and splenic vein were observed for light mic：roscopy. Samples were made into ultrathin-slices again. The ultrastructural changes of endothelial cells were observed under transmission electron microscopy. Results In varicose great saphenous veins and diseased splenic veins, the nuclear architecture of endothelial cells in vasa vasorum were integrity and the distribution of chromatin were normal. In some mitochondria, the trachychromatic grotmdplasm, undefined and ruptured cristae were found. Conclusion Under high hydrostatic pressure and hypoxia conditions, the ultrastructure of vasa vasorum endothelial cells betweern the great saphenous vein and the splenic vein may appear remodeling phenomenon, and both changes are similar.
出处
《中国普外基础与临床杂志》
CAS
2014年第10期1241-1244,共4页
Chinese Journal of Bases and Clinics In General Surgery
关键词
静脉曲张
病脾静脉
滋养血管
内皮细胞
超微结构
Varicose vein
Diseased splenic vein
Vasa vasorum
Endothelial cell
Ultrastructure
