摘要
目的 分析安徽省2013年分离的5株人感染H7N9禽流感病毒全基因组特征.方法 从美国国家生物技术信息中心（NCBI）和全球禽流感基因共享数据库（GISAID）中下载具有代表性的H7N9、H7N3、H7N7和H9N2等毒株序列,运用分子生物信息学软件分析病毒全基因组特征.结果 我省流行的H7N9病毒HA基因与A/duck/Fujian/6390/2010（H7N3）相似度最高,NA基因与A/northern shoveler/Hong Kong/MPL133/2010（H2N9）株相似度最高,6个内部基因片段与中国北京、香港、湖南、江苏地区分离的H9N2毒株相似度接近.氨基酸序列比对发现NS1蛋白218～230位氨基酸缺失、M2蛋白的N31S突变、HA蛋白的G186V、Q226L突变以及NA蛋白69～73位的删除,并且我省人感染H7N9病毒均带有PB2的E627K突变,同时PA-100A、PA-356R、PA-409N这些易感人类的特征氨基酸也在本次流行的H7N9病毒中发现;此外HA蛋白裂解位点仅有1个碱性氨基酸、糖基化位点高度保守以及未发现NA蛋白R294K突变也是我省H7N9病毒主要特征.结论 我省人感染H7N9病毒与中国其他省份流行株高度同源,该病毒获得跨种传播、毒力增强、耐药等能力均与病毒蛋白功能域有关.
To understand genomic features of 5 avian influenza virus A （HTN9） cases isolated from Anhui Province, China, the genome sequences of influenza virus H7Ng, H7N3, H7N7 and H9N2 subtypes were downloaded from database of NCBI （The National Center for Biotechnology Information） and GISAID （Global Initiative on Sharing All Influenza Data）. Ge- nomic features were analyzed with molecular bioinformatics software. In H7N9, the highest homology was found in HA gene with A/duck/Fujian/6390/2010（H7N3）, and NA gene with A/northern shoveler/Hong Kong/MPL133/2010（H2N9）. Six genes encoding for internal proteins shared high identity with HgN2 avian influenza isolated from Beijing, Hong Kong, Hunan, and Jiangsu in China. The amino acid sequence alignment result showed that 218 to 230 amino acids were deleted in NS1 ; N31S was mutated in M2~ G186V and Q226L were mutated in HAs 69 to 73 amino acids were deleted in NA. In addition, E627K was mutated in PB2 in H7N9 virus isolated from human. A few amino acids with human features including PA-100A and PA- 356R; PA-409N were also found in this HTN9 virus. The other main features included single basic amino acid of HA cleavage sequence, high conservation of the glycosylation sites, and non-mutation of R294K in NA protein. In conclusion, high homolo- gy was existed in avian influenza virus （H7N9） between Anhui Province and other epidemic provinces. The characterization of cross-species transmission, increased virulence, and drug-resistance were related with functional domains of viral ;roteins.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2014年第9期913-918,共6页
Chinese Journal of Zoonoses
