摘要
目的探索陈元膏摩对膝骨性关节炎(knee osteoarthritic,KOA)的作用机制及临床意义,为该法在临床中的应用提供理论依据。方法 52只清洁级雄性SD大鼠,分别为空白组、模型组、凡士林手法组、陈元膏摩组,用管型石膏将模型组、凡士林手法组、陈元膏摩组三组大鼠的左下肢膝关节固定8周,形成KOA中期改变的动物模型。空白组、模型组两组不做干预,凡士林手法组施以凡士林膏结合手法治疗,陈元膏摩组施以陈元膏结合摩法治疗,治疗8周;疗程结束后,通过免疫组织化学方法测量大鼠血清中肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)、转化生长因子β(transforming growth factor-beta,TGF-β)含量水平;HE染色法观察四组大鼠关节软骨基质的病理变化程度并进行Mankin评分。结果凡士林手法组、陈元膏摩组两组的Mankin评分及血清TNF-α浓度均低于模型组,且陈元膏摩组与凡士林手法组相比TNF-α浓度较低(P<0.05);凡士林手法组、陈元膏摩组两组的血清TGF-β浓度均高于模型组,且陈元膏摩组与凡士林手法组相比TGF-β浓度较高(P<0.05)。结论陈元膏摩可以减轻KOA关节软骨基质的破坏及关节病损,早期应用可抑制炎症细胞因子TNF-α的破坏作用,促进转化生长因子TGF-β的修复。
Objective To explore the mechanism of action and clinical significant of Chenyuan Ointment for controlling and treating knee osteoarthritis,and to provide the theory evidence for the clinical application. Methods Selected 52 apinoid SD rats and divided into 4 groups randomly ： —normal group,model group,Calligraphy control group and Chenyuan cream group. Fixed every rat＇ left knee-joint of model group,Calligraphy control group and Chenyuan cream group by closed-plaster method for 8 weeks to induce the knee osteoarthritis（ KOA） model. After 8 weeks fixation,the rats in normal group、and model group without intervention,the rats in Calligraphy control and Chenyuan cream groups were given Chenyuan cream with manipulation and Vaseline with manipulation on a daily basis. After 8 weeks,tdetected the serum levels of TNF-α and TGF-β,HE staining,observed the pathological changes of articular cartilage in each groups and Mankin scoree. Results Compared with model group,Calligraphy control and Chenyuan cream groups showed lower Mankin score and the serum levels of TNF-α（ P〈0. 01）,Between Calligraphy control and Chenyuan cream groups,Chenyuan cream group showed the lower serum levels of TNF-α（ P〈0. 05）;Compared with model group,Calligraphy control group、Chenyuan cream group showed higher the serum levels of TGF-β（ P〈0. 01）,Between Calligraphy control group and Chenyuan cream group,Chenyuan creamgroup showed the higher serum levels of TGF-β. Conclusion Chenyuan Ointment rubbing can alleviate the joint cartilage destruction and the joint damage in KOA,which is achieved through reducing the destruction of inflammatory cellular factor TNF-α and promoting the repair of transforming growth cellular factor TGF-β.
出处
《环球中医药》
CAS
2014年第10期748-751,共4页
Global Traditional Chinese Medicine
基金
新疆名医名方与特色方剂学实验室开放课题项目(XJDX0910-2011-4)
新疆医科大学2012年科研创新基金(XJC2012108)
关键词
膝骨性关节炎
肿瘤坏死因子α
转化生长因子Β
软骨基质
Osteoarthritis of knee join
Tumor necrosis factor alpha
Transforming growth factor-beta
Mesochondrium
