摘要
目的:探讨c-Myc、k-Ras、p16蛋白在胆囊癌细胞中的表达及临床意义。方法:收集20例胆囊癌、50例慢性胆囊炎和20例正常胆囊组织标本,用免疫组化法检测c-Myc、k-Ras、p16蛋白的表达,分析三种蛋白表达与胆囊癌的临床病理特征关系。结果:c-Myc、k-Ras蛋白定位于细胞质和细胞核中,在胆囊癌中的表达程度明显高于慢性胆囊炎和正常胆囊,差异有统计学意义(P<0.01)。p16定位于胞质,在胆囊癌中的表达程度明显低于正常胆囊和慢性胆囊炎,差异有统计学意义(P<0.01)。c-Myc表达程度与肿瘤大小、分化和淋巴结转移密切相关(P<0.05);k-Ras表达程度与肿瘤大小、侵犯深度和淋巴结转移密切相关(P<0.05)。结论:c-Myc、k-Ras、p16蛋白在胆囊癌细胞中呈异常表达,其表达与临床病理特征相关,可能在胆囊癌发生发展中起有重要作用。
Objective：To investigate the expressions of c-Myc, k-Ras and p16 protein in the gallbladder carcinoma cells and their clinical implication. Methods：The expressions of c-Myc, k-Ras and p16 protein were detected in 20 cases of gallbladder carcinoma, 50 cases of chronic cholecystitis and 20 cases of normal gallbladder tissue specimens with the immunohistochemical method. The relationships of the expressions of three kinds of proteins with the clinical pathological characteristics were assayed. Results：The c-Myc and k-Ras expressions were localized in the cytoplasm and nucleus of tumor cells, and the expressions in gallbladder carcinoma were signiifcantly higher than those in chronic cholecystitis and the normal gallbladder, whose difference had the statistical signiifcance （P〈0.01）. The location of p16 protein was found in the cytoplasm. The expression degrees in the gallbladder carcinoma were obviously lower than those in the normal gallbladder and chronic cholecystitis, whose difference had the statistic signiifcance （P〈0.01）. The expression degree of c-Myc was signiifcant correlation with the size of the tumor, poor differentiation, and lymphatic metastasis （P〈0.05）. The expression degree of k-Ras was significant correlation with the size of the tumor, depth of invasion, and lymphatic metastasis （P〈0.05）. Conclusion：The expressions of c-Myc, k-Ras, and p16 protein present abnormal in the gallbladder carcinoma cells and their expressions are correlated with the clinical pathological characteristics which may probably play an important role in the occurrence and development of the gallbladder carcinoma.
出处
《上海医药》
CAS
2014年第18期27-29,共3页
Shanghai Medical & Pharmaceutical Journal
基金
上海市卫生局科研资助项目(2010143)
