摘要
目的：建立毛菊苣总倍半萜滴丸中山莴苣素的溶出度测定方法.方法：采用桨法测定体外溶出度,通过单因素试验筛选溶出介质和转速,利用HPLC测定毛菊苣总倍半萜滴丸中山莴苣素的溶出度,流动相甲醇（A）-0.2％甲酸（B）梯度洗脱（0 - 30 min,28％- 72％ A）,检测波长256 nm.结果：选择含0.2％十二烷基硫酸钠的人工胃液为溶出介质,转速100r·min-1,山莴苣素线性范围0.015 -0.075 g·L-1（r=0.999）,平均回收率101.04％ （RSD 1.38％）.样品30 min时体外溶出度均＞80％.结论：本方法简便、准确、可靠,适用于毛菊苣总倍半萜滴丸的溶出度测定.
Objective：To establish a method for determining dissolution of lactucin in total sesquiterpenes from Cichorium glandulosum dropping pills.Method：Paddle method was adopted in which artificial gastric juice was taken as solvent with a rotating speed of 100 r · min-1 Dissolution of lactucin was determined by HPLC and dissolution curves were drawn,mobile phase is made up of methanol （A）-0.2％ formic acid （B） for gradient elution （0-30 min,28％-72％ A） and detection wavelength was set at 256 nm.Result：Lactucin was linear over the range of 0.015-0.075 g ·L-1 with r of 0.999 and average recovery was 101.04％ with RSD of 1.38％.Taking artificial gastric juice containing 0.2％ sodium dodecyl sulfate as dissolution medium,dissolution of samples were all more than 80％ in 30 min.Conclusion：This method is simple,accurate and reliable for dissolution determination of total sesquiterpenes from Cichorium glandulosum dropping pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第21期21-24,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81303307)
关键词
毛菊苣
总倍半萜
溶出度
山莴苣素
Cichorium glandulosum
total sesquiterpenes
dissolution
lactucin
