摘要
[目的]建立同时测定电子烟烟液中甲醛、乙醛、丙酮、丙烯醛、丙醛、巴豆醛、2-丁酮和丁醛8种挥发性羰基化合物含量的高效液相色谱法。[方法]电子烟烟液经2,4-二硝基苯肼溶液衍生后,衍生物直接用HPLC测定。选用DIONEX Acclaim Explosive E2色谱柱（250 mm×4.6 mm,5μm）,以乙腈和水为流动相进行梯度洗脱,通过紫外检测器检测烟液中羰基化合物。[结果]8种羰基化合物的DNPH衍生物在一定浓度范围内与峰面积线性关系良好,相关系数均达到0.999以上,3个加标水平的平均回收率在96.4%~101.1%,相对标准偏差（RSD）小于3.26%,检出限（LOD）为0.009~0.035 mg/L。[结论]该方法前处理简单,灵敏度高,精密度及准确度好,适用于电子烟烟液中羰基化合物的检测。
[ Objective] A high performance liquid chromatography （HPLC） method was established to determine the eight volatile carbonyl compounds （formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone, and butyraldehyde） in the refill liquids for electronic cigarettes. [ Method] After carbonyl compounds in the refill liquids were reacted with 2, 4-dinitrophenylhydrazine （DN- PH） in an acidic medium to form 2,4-dinitrophenylhydrazone derivatives, which were separated on DIONEX Acclaim Explosive E2 column （250 mm × 4.6 mm, 5 μm） under the gradient elution with acetonitrile and water as mobile phase, and analyzed with a ultraviolet detector. [ Result] The eight volatile carbonyl compounds had good correlation coefficients （R2 more than 0. 999 ） in corresponding concentration ran- ges, the average spiked recoveries ranged from 96.4% to 101.1% with a relative standard deviation lower than 3.26% ,and the limit of detec- tion is between 0.009 -0.035 mg/L. [ Conclusion] The developed method featured simple pretreatment, high sensitivity, good accuracy and precision, and it is successfully applied for the simultaneous determination of the eight carbonyl compounds in refill liquids.
出处
《安徽农业科学》
CAS
2014年第31期11072-11074,11082,共4页
Journal of Anhui Agricultural Sciences
关键词
电子烟
烟液
羰基化合物
2
4-二硝基苯肼
高效液相色谱
Electronic cigarettes
Refill liquids
Carbonyl compounds
2,4-dinitrophenylhydrazine
High performance liquid chromatography
