摘要
目的:建立测定人参归脾丸中黄芪甲苷的高效液相色谱分析方法。方法采用高效液相色谱法,色谱柱 Agilent TC C18柱(4.6 mm×250 mm,5μm),柱温25℃,流动相:甲醇-水(75:25),流速1.0 mL·min-1; ELSD 参数:漂移管温度90℃载气流量1.6 L·min-1。结果黄芪甲苷在37.5-600μg·mL-1内线性关系良好,平均回收率为98.8%, RSD 为3.4%。结论该方法准确、简便、专属性好,可用于人参归脾丸中黄芪甲苷成分的含量测定,为人参归脾丸的质量控制提供依据。
Objective To establish an HPLC to determine the content of astragaloside in Renshenguipi pills. Methods The HPLC-ELSD was used with Agilent TC C18 column (4. 6 mm×250 mm, 5 μm) at 25 ℃ . The mobile phase was methanol and water (75 :25) with a flow rate at 1. 0mL·min-1 . The temperature of the drift tube in ELSD was 90 ℃ and the flow rate of dry air was 1. 6 L·min-1 . Results Astragaioside showed good regression ( r = 0. 999 0) at 37. 5 - 600 μg · mL-1 . The average recovery was 98. 8% . RSD was 3. 4% . Conclusion The method is accurate, simple and reproducible, and can be used for the content determina-tion of astragaloside in components of ginseng Guipi pills, providing basis for the quality control of Renshenguipi pills.
出处
《辽宁医学院学报》
CAS
2014年第5期9-11,I0003,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly