摘要
目的探讨番茄红素对饮水性氟染毒小鼠氧化应激损伤与神经行为影响及可能机制。方法成年雄性C57BL/6小鼠60只,随机分为对照组、染氟组、番茄红素组、番茄红素低、中、高剂量干预组,每组10只;连续处理6个月后,氟离子选择电极法测定尿氟、血氟和脑氟含量;化学比色法检测血液和海马组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活力;水迷路实验分析小鼠神经行为学改变。结果与对照组比较,染氟组小鼠体重下降,尿氟、血氟和脑氟含量[分别为(3.76±1.60)、(19.26±0.62)、(1.19±0.12)mg/kg]升高(P<0.05),血液和海马组织中MDA含量显著增加、SOD、GSH-Px和CAT酶活力下降(P<0.05);与染氟组比较,番茄红素高剂量干预组尿氟、血氟和脑氟含量[分别为(2.06±0.47)、(13.40±0.35)、(0.52±0.21)mg/kg]明显下降(P<0.05),血液和海马组织中MDA含量降低、SOD、GSH-Px和CAT酶活力升高(P<0.05);与对照组比较,染氟组小鼠平均逃逸时间和靶象限活动时间[分别为(20.84±0.72)、(16.11±0.77)s]延长(P<0.05);与染氟组比较,番茄红素高剂量干预组小鼠平均逃逸时间和靶象限活动时间[分别为(14.01±0.82)、(7.38±0.37)s]下降(P<0.05)。结论番茄红素能有效降低饮水性氟染毒小鼠氧化应激损伤及提高小鼠学习记忆能力,对饮水性氟中毒具有一定保护作用。
Objective To investigate the relationship between lycopene exposure and neurobehavior and its mechanism in mice exposed to drinking water fluoride.Methods Sixty adult mice were randomly divided into six groups（ten animals in each group）：control group,lycopene group,sodium fluoride（NaF）group,NaF plus low,medium,high dose lycopene groups.After 6 months’treatment,fluoride ion selective electrode method was used to determine the fluorine concentrations of urine,blood and hippocampus.Spectrophotometry was used to determine the activities of superoxide dismutase（SOD）,glutathione peroxidease（GSH-PX）,catalase（CAT）,and the contents of malondialdehyde（MDA）in serum and hippocampus.We also examined neurobehavioral abnormalities with WT-200 water maze.Results Compared with the control group,NaF administration induced a decrease in body weight markedly（P〈0.05）;the fluorine concentrations of urine,blood,and hippocampus were 2.06±0.47,13.40±0.35,and 0.52±0.21 mg/kg and increased markedly（P〈0.05）.At the same time,NaF administration induced oxidative stress indicated by elevated levels of MDA in serum and brain tissues（P〈0.05）.Moreover,significantly decreased activities of SOD,GSH-PX,CAT were observed in the tissues examined（P〈0.05）.Compared with NaF group,the fluorine concentrations of urine,blood,and hippocampus（2.06±0.47,13.40±0.35,and 0.52±0.21mg/kg）decreased in NaF plus high dose lycopene group（P〈0.05）.The induced oxidative stress and the alterations in antioxidant system were normalized by the oral administration of medium and high dose lycopene treatment（P〈0.05）.Compared with the control group,the escape time and activity time were 20.84±0.72 s and16.11±0.77 s（P〈0.05）in NaF group.Compared with NaF group,the escape time and activity time（14.01±0.82 s and7.38±0.37s）decreased in high dose lycopene group（P〈0.05）.Conclusion Lycopene administration could minimize toxic effects of fluoride,indicating its free radical scavenging and powerful antioxidant activities.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第11期1391-1394,共4页
Chinese Journal of Public Health
基金
浙江省自然科学基金(Y2110388)
浙江省科技厅公益性应用研究项目(Y2011C37091)
关键词
番茄红素
氟染毒
氧化性应激
神经行为
小鼠
lycopene
fluoride exposure
oxidative stress
neurobehavior
mouse
