摘要
目的:探讨不同光照条件对果蝇脑部神经递质5-HT和DA含量变化的影响。方法:以7日龄雌果蝇为研究对象,选取5-HT和DA为指标,利用酶联免疫吸附测定(ELISA)技术,考察12 h/12 h明暗交替和24 h持续黑暗环境下,7日龄果蝇在不同时间点(16:00、19:00、22:00、1:00、4:00和7:00)脑部5-HT和DA的含量变化。结果:明暗交替组果蝇脑部5-HT和DA含量呈现相同的变化趋势;持续黑暗组果蝇脑部5-HT含量和DA含量呈现不同的变化趋势。结论:不同光照条件对果蝇脑部5-HT和DA含量的影响不同。
Objective： The purpose of this study was to investigate the effects of different illumination condition on the content of brain 5- HT and DA in 7- day- old female drosophilas.Methods：Seven- day- old female drosophilas were selected for the observation using enzyme- linked immunosorbent assay（ ELISA）,the dissertation observes the content changes of 5- HT and DA in drosophila’s brain of different time（16：00,19：00,22：00,1：00,4：00 and 7：00） in 12 h /12 h alternating light and dark and 24 h constant darkness environment and investigates the effects.Results：The content of 5- HT and DA of the drosophila brain in 12 h /12 h alternating light and dark group shows the same change trends; and the continuous dark group shows different change trends.Conclusion：There are different influences on content of 5- HT and DA of drosophila brain in different light conditions.
出处
《中国药物依赖性杂志》
CAS
CSCD
2014年第5期358-362,共5页
Chinese Journal of Drug Dependence
基金
国家自然科学基金面上项目(81274114)
黑龙江省博士后资助(LBH-Z13193)
黑龙江中医药大学优秀创新人才支持计划(2012RCL03)
