摘要
目的观察不同剂量葡萄籽提取物原花青素对三氧化二砷(As2O3)所致雄性小鼠肝脏毒性干预效果,研究其抗氧化损伤作用机制及其干预效果的差异。方法按体重分层将50只雄性昆明种小鼠随机分为5组:对照组(生理盐水0.9%)、As2O3组(4 mg/kg·bw)、和As2O3(4 mg/kg·bw)+GSPE低(100 mg/kg·bw)、中(200 mg/kg·bw)、高(400 mg/kg·bw)剂量组,每组10只,连续灌胃5周;称量小鼠体重、肝重,并计算其肝脏系数;测定肝脏组织的天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、T-AOC等指标,采用单因素方差分析对比组间差异。结果 As2O3组体重、GSH含量、T-AOC水平均低于对照组,肝重、肝脏系数、ALT、AST活力、MDA含量均高于对照组(P均<0.05);As2O3+GSPE(200、400)mg/(kg·bw)剂量组体重均高于As2O3组(P均<0.05);As2O3+GSPE(100、200、400)mg/(kg·bw)剂量组肝重、肝脏系数、ALT、AST活力、MDA含量均低于As2O3组(P均<0.05);GSH含量、T-AOC水平均高于As2O3组(P均<0.05)。As2O3+GSPE低剂量组小鼠体重比As2O3+GSPE中剂量组低(P<0.05);As2O3+GSPE低、中、高剂量组GSH含量(137.17±34.70)μmol/g prot、(198.02±41.28)μmol/g prot、(272.06±35.85)μmol/g prot、T-AOC水平(5.33±0.76)U/mL、(7.07±0.83)U/mL、(11.54±1.86)U/mL依次增高(P<0.05)。结论 GSPE可能通过抗氧化损伤作用拮抗As2O3致雄性小鼠的肝脏毒性,高剂量GSPE干预效果较好。
Objectives To observe the intervention of grape seed procyanidin extract（GSPE） on liver toxicity in mice induced by arsenic trioxide（As2O3） and to evaluate the antioxidant mechanism of GSPE. Methods Fifty healthy male KM mice were randomly divided by body weight into 5 groups： a control group（saline,0. 9% NaCl）,a As2O3（4 mg/kg·bw） group,and three As2O3（4 mg/kg·bw） ＋GSPE groups with high dose（400 mg/kg·bw）,middle dose（200 mg /kg·bw） and low dose（100 mg /kg·bw） of GSPE. The 10 mice in each group were treated by gastric perfusion for 5 weeks; and the body weight,liver weight and liver index were measured. The activities and levels of AST,ALT,T-AOC,MDA and GSH in liver tissue were measured by test kits. Results The content of GSH,the body weight,the level of T-AOC in As2O3 treated groups were significantly lower than the control group（P 〈 0. 05）. The liver weight,the liver index,the activities of ALT and AST,the content of MDA in As2O3 treated groups were significantly higher than the control group（P 〈 0. 05）. The body weight of As2O3＋GSPE groups with high and middle dose of GSPE was significantly higher than the As2O3 group（P 〈 0. 05）. The liver weight,the liver index,the activities of ALT and AST,the content of MDA in all As2O3＋ GSPE groups were significantly lower than the As2O3 group（P 〈 0. 05）. The content of GSH,the level of T-AOC in all As2O3＋GSPE groups were significantly higher than the As2O3group（P 〈 0. 05）. The body weight of As2O3＋ low GSPE dose group was significantly lower than the As2O3＋ middle GSPE dose group（P 〈 0. 05）; The content of GSH in As2O3＋ high GSPE dose group was significantly higher than the As2O3＋ low and middle dose GSPE group（P 〈0.05）. Conclusions The liver toxicity induced by arsenic trioxide might be reduced by GSPE in the way of antioxidant mechanism. Higher dose of GSPE was more effective in the inhibition of liver toxicity.
出处
《环境卫生学杂志》
北大核心
2014年第5期430-433,437,共5页
JOURNAL OF ENVIRONMENTAL HYGIENE
基金
兵团重点领域科技攻关项目(项目编号2014BA039)
石河子大学高层次人才项目(项目编号:RCZX201112)
石河子大学国家级大学生创新创业训练计划项目(项目编号:201310759069)
关键词
葡萄籽原花青素
三氧化二砷
雄性小鼠
肝脏毒性
grape seed procyanidin extract（GSPE）
arsenic trioxide
male mice
liver toxicity
