摘要
建立了高效液相色谱法同时测定三七提取物中三七皂苷R1与人参皂苷Rg1、Rb1含量的方法.采用高效液相色谱仪,Agilent ODS-C18（5μmn,4.6mm×250mm）液相色谱柱,流动相为乙腈-水梯度洗脱,流速为1.0 mL/mim,检测波长为203 nm,柱温为35℃.结果表明,三七皂苷R1在度15.29～244.60 mg/L的范围内（r=0.9998）,人参皂苷Rg1在14.30～228.90 mg/L范围内（r=1.0000）,人参皂苷Rb1在13.13～210.11 mg/L范围内（r=1.0000）,线性关系良好.三七皂苷R1和人参皂苷Rg1、人参皂苷Rb1平均加标回收率分别为99.54％、101.61％、102.99％,峰面积相对标准偏差RSD（n=6）分别为1.67％、0.93％、0.92％.该方法简便、准确,重现性好,可以对三七提取物中三七皂苷R1与人参皂苷Rg1、Rb1进行定量分析.
A high performance liquid chromatography method was established for the simultaneous determination of notoginsenoside Ri and ginsenoside Rg1 and Rb1 in Panax notoginseng. The HPLC separation was carried out on Agilent ODS- C18 column （5 μm, 4.6 mm×250 mm）, the mobile phases were acetonitrile- water with gradient elution, the detection wavelength was 203 nm, the flow rate was 1.0 mL/min and the column temperature was 35 ℃. The results showed good linearity in the range of 15.29-244.60mg/L for notoginsenoside R1（r=0.9998）, 14.30-228.90 mg/L and 13.13-210.11 mg/L for ginsenoside Rg1 （r=1.0000） and ginsenoside Rbt（r=1.0000）respectively. The average recoveries of notoginsenoside R1 and ginsenoside Rgl and Rb1 were 99.54 %, 101.61% and 102.99 %, and the relative standard deviation （n=6） were 1.67 %, 0.93 % and 0.92 % respectively. The method is simple, accurate, reproducible and suitable for simultaneous quantitative measurement of notoginsenoside R1 and ginsenoside Rg1 and Rb1 in Panax notoginseng.
出处
《广东化工》
CAS
2014年第19期195-196,共2页
Guangdong Chemical Industry
