摘要
实验研究了鸡血细胞密度、PEG浓度、PEG作用时间、鸡血细胞悬液放置时间、钙离子浓度等因素对鸡血细胞体外融合的影响.结果表明:细胞密度为1×107个/mL,PEG浓度为50%,PEG作用时间为10min,细胞悬液放置时间为10min,钙离子浓度为50.0mmol/L时细胞融合率最高.
The effects of chicken blood cell density ,PEG concentration ,PEG duration ,standing time of chicken blood cell suspension ,calcium ion concentration on cell fusion in vitro were studied .The results showed as follows ：cell fusion rate was the highest when cell density was 1 ＆#215; 107/mL ,PEG concentration was 50% ,PEG duration was 10 minutes ,standing time of chicken blood cell suspension was 10 minutes ,calcium ion concentration was 50.0 mmol/L .
出处
《周口师范学院学报》
CAS
2014年第5期96-99,共4页
Journal of Zhoukou Normal University
关键词
鸡血细胞融合
聚乙二醇
细胞密度
细胞悬液放置时间
钙离子浓度
chicken blood cell fusion
PEG
cell density
standing time of cell suspension
calcium ion concentration
