摘要
目的 探讨血液肝癌细胞相关甲胎蛋白（AFP） mRNA阳性与肝癌患者术后肿瘤复发或转移的关系.方法 应用巢式RT-PCR检测62例原发性肝癌患者术前1天周围静脉血液中AFPmRNA,术后随访3年.结果 24例术前血液检测AFPmRNA阳性,38例患者术前AFPmRNA呈阴性.术前血AFPmRNA阳性的患者术后1、2、3年肿瘤复发率分别为58.3％ （14/24）、75.0％（18/24）、100.0％ （24/24）;而术前血AFPmRNA阴性的患者术后1、2、3年肿瘤复发率则分别为23.6％ （10/38）、42.1％（16/38）、68.4％（26/38）,两者比较复发率差异具有统计学意义（P＜0.01）.术前血AFPmRNA阳性的患者术后3年有8.3％ （2/24）发生肺转移;而阴性患者术后3年内无一发生肺或其他远处转移（0.0％,0/38;P＜0.01）.结论 术前血液AFPmRNA阳性的肝癌患者较AFPmRNA阴性的肝癌患者术后更容易产生复发或远处转移.
Objective To study the correlation between positive peripheral blood hepatocellular carcinoma （HCC） cells-associated AFPmRNA and HCC recurrence / distant metastasis after HCC resection.Methods We studied blood AFPmRNA in 62 HCC patients using nested RT-PCR 1 day before hepatectomy,and followed these patients up for 1 to 3 years after surgery.Results 24 HCC patients were tested AFPmRNA positive before surgery,while 38 HCC patients were tested negative.Of the 24 patients who were tested positive for AFPmRNA,14 relapsed （14/24,58.3％） within the first year of surgery,4 in the second year （18/24,75.0％）,the remaining 6 in the third year （24/24,100％）.For the 38 patients who were tested AFPmRNA negative,10 patients （10/38,26.3％） relapsed within the first year,6 in the second year （16/38,42.1％）,and 10 in the third year （26/38,68.4％,P ＜ 0.01）.There was significant difference between patients who were tested AFPmRNA positive and AFPmRNA negative in relation to tumor recurrence （P ＜ 0.01）.Among 24 patients who were tested AFPmRNA positive,2 patients developed lung metastasis in the third year （2/24,8.3％）.There was no patient who developed lung metastasis or distal metastasis in the third year in the patients who were tested AFPmRNA negative （0/38,0.0％,P ＜ 0.01）.Conclutions HCC patients who were tested AFPmRNA positive before operation were more prone to develop HCC recurrence or distal metastasis when compared with those patients who were tested AFPmRNA negative.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2014年第10期697-699,共3页
Chinese Journal of Hepatobiliary Surgery
基金
国家自然科学基金（81372461）
