摘要
目的 评价夫西地酸对痤疮丙酸杆菌的体外抗菌活性.方法 上海华山医院2013年3-9月收集的50株痤疮丙酸杆菌,按美国临床实验室标准化研究所推荐的琼脂稀释法测定夫西地酸及其他抗菌药的最低抑菌浓度（MIC）.数据分析采用WHONET 5.4软件.结果 夫西地酸对痤疮丙酸杆菌的MIC50和MIC90分别为0.5和1.0 mg/L,敏感率为90％;莫西沙星与夫西地酸大致相仿,敏感率为90％;克林霉素和红霉素的敏感率分别为54％和46％,MIC90值均＞128 mg/L;对甲硝唑耐药.在药物质量浓度为128 mg/L时,克林霉素的累积抑菌率达到70％,红霉素只有48％,而夫西地酸在2 mg/L时的累积抑菌率已达到100％.结论 夫西地酸对临床分离的痤疮丙酸杆菌具有良好的体外抗菌活性.
Objective To evaluate the in vitro antibacterial activity of fusidic acid against P.acnes.Methods Fifty strains of P.acnes were clinically isolated from Huashan Hospital,Fudan University from March to September 2013.The minimum inhibitory concentrations （MICs） of several antibacterial agents including fusidic acid against these P.aches isolates were determined by using the agar dilution method according to the recommendations of Clinical and Laboratory Standards Institute （CLSI）.Data were analyzed using the WHONET 5.4 software.Results Among the 50 P.acnes isolates,90% were sensitive to fusidic acid,90% to moxifloxacin,54% to clindamycin,46% to erythromycin,but 100% were resistant to metronidazole.The minimum concentration required to inhibit the growth of 50% organisms （MIC50） and 90% organisms （MIC90） were 0.5 and 1.0 mg/L respectively for fusidic acid,whereas clindamycin and erythromycin both showed higher MIC90 values （〉 128 mg/L）.At the concentration of 128 mg/L,clindamycin inhibited the growth of 70% of the P.acnes isolates,and erythromycin inhibited the growth of 48% of them,while the growth of all the isolates was inhibited by fusidic acid at 2 mg/L.Conclusion Fusidic acid exhibits excellent antibacterial activity against clinical isolates of P.acnes in vitro.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2014年第11期823-825,共3页
Chinese Journal of Dermatology
基金
国家科技部“国家科技重大新药创制”专项资助（2012ZX09303004-001）
2013年中西医结合（CAIM）-澳美中国痤疮研究基金
关键词
丙酸杆菌
痤疮
梭链孢酸
微生物敏感性试验
Propionibacterium acnes
Fusidic acid
Microbial sensitivity tests
