摘要
目的:探讨miR-150和miR-134在结直肠癌与结直肠腺瘤中的表达及意义。方法:采用实时定量荧光PCR(qRT-PCR)检测40例结直肠癌组织及其癌旁正常组织与29例结直肠腺瘤组织中miR-150和miR-134表达,并分析两者与结直肠癌临床病理因素之间的关系。结果:与癌旁正常组织比较,miR-150在腺瘤组织中表达明显升高,而在癌组织中表达明显降低(均P<0.05);miR-134在腺瘤组织中表达明显降低(P<0.05),但在癌组织中表达差异无统计学意义(P>0.05);miR-150表达水平与结直肠癌的组织学类型及分化程度有关(P=0.033,P=0.041);miR-134表达水平与结直肠癌的各项临床病理因素均无明显关系(均P>0.05)。结论:miR-150在结直肠癌中表达下调,提示其可能有潜在的抑癌作用,miR-150和miR-134在结直肠腺瘤中的表达均发生异常,提示两者均可能与结直肠腺瘤的发生密切相关。
Objective： To investigate the expression of miR-150 and miR-134 in colorectal cancer and colorectal adenoma and evaluate the significance. Methods： The miR-150 and miR-134 expressions in 40 tissue specimens of colorectal cancer along with their adjacent normal mucosa and 29 tissue specimens of colorectal adenoma were determined by real-time quantitative reverse transcription-PCR （qRT-PCR） Results： Compared with normal mucosal tissue, the miR-150 expression was significantly adenoma, while was significantly decreased in colorectal cancer （both P〈0.05）; the increased in colorectal miR- 134 expression was significantly decreased in colorectal adenoma （P〈0.05）, but showed no obvious difference in colorectal cancer （P〉0.05）. The miR-150 expression level was significantly related to the histological type and degree ofdifferentiation of colorectal cancer （P=0.033, P=0.041）, while the miR-150 expression level was irrelevant to any of the clinicopathologic factors ofcolorectal cancer （all P〉0.05）. Conclusion： milk-150 expression is down-regulated in colorectal cancer, suggesting that miR-150 may probably have a potential anti-tumor activity; both miR-150 and miR-134 expression are abnormal in colorectal adenoma, suggesting that they may be closely related to the occurrence of colorectal adenoma.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2014年第10期1349-1354,共6页
China Journal of General Surgery
基金
国家自然科学基金资助项目(81072034)
河北省自然科学研究计划资助项目(C2011206103)
河北省国际合作资助项目(12396105D)
关键词
结直肠肿瘤
癌
腺瘤
微RNAS
Colorectal Neoplasms
Carcinoma
Adenoma
MicroRNAs
