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甲状腺非典型腺瘤中DAPI核染色、Ki-67表达及意义 被引量：1

DAPI nuclear stain and Ki-67 expression in atypical thyroid adenoma and their clinical significance
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摘要 目的探讨4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole,DAPI)细胞核染色、Ki-67在甲状腺非典型腺瘤(atypical thyroid adenoma,ATA)中的表达及临床意义。方法采用免疫组化EnVision两步法和免疫荧光染色法分别检测ATA、甲状腺滤泡癌(follicular thyroid carcinoma,FC)中Ki-67增殖指数及DAPI核染色情况。结果 DAPI在低、高核级ATA组织中的阳性率分别为12.50%(2/16)、16.13%(5/31),两组相比差异无统计学意义(χ2=0.01,P>0.05);其在对照组FC中的阳性率为85.71%(6/7),两组相比差异有统计学意义(χ2=13.17,P<0.01);Ki-67在低、高核级ATA组织中的阳性率分别为6.25%(1/16)、6.45%(2/31),二者相比差异无统计学意义(χ2=0.36,P>0.05);而在对照组FC中的阳性率为85.71%(6/7),二者相比差异有统计学意义(χ2=22.19,P<0.01);Ki-67、DAPI表达与ATA患者年龄、结节数量、肿块大小无关。结论联合检测Ki-67和DAPI可能对ATA与FC的鉴别诊断有一定价值。Ki-67、DAPI与ATA核级别、患者年龄、肿块大小和结节数量无关。 Purpose To study the 4＇, 6-diamidino-2-phenylindole （DAPI） nuclear staining and Ki-67 expression in atypical thyroid adenoma （ATA） and their clinical parameters. Methods Ki-67 proliferative activity and DAPI nuclear staining in ATA and follicular carcinoma （FC） were detected with immunohistochemical EnVision two-step method and immunofluorescence staining respectively. Results DAPI positive rate in ATA with low and high nuclear grade was 12.50% （2/16） and 16.13 % （5/31 ）, respectively; there was no significant difference between them （ ~2 = 0. 01, P 〉 0. 05 ）. the positive rate of DAPI in FC was 85.71% （6/7）, which higher than that in ATA （ ~2 = 13. 17, P 〈 0. 01 ）. Ki-67-positive rate in ATA with low and high nuclear grade was 6.25% （1/16） and 6.45% （2/31） with no significant difference between them （ χ2 = 0. 36, P 〉 0.05 ）. Ki-67 expression in FC was 85.7 1% （6/7） which was statistically significant as compared to ATA （χ2 = 22. 19, P 〈 0. 01 ）. Ki-67 and DAPI in ATA were unrelated to age, nod- ule number, and tumor size. Conclusion The detection of Ki-67 and DAPI expression may be helpful for the differential diagnosis of ATA and FC, which may be not related to nuclear grade, age, tumor size and nodular number.
作者 邢益祥 孟刚
机构地区 安徽医科大学基础医学院病理学教研室 合肥华澳临床医学检验所病理实验室
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2014年第10期1117-1121,共5页 Chinese Journal of Clinical and Experimental Pathology
关键词 甲状腺肿瘤 非典型腺瘤 KI-67 DAPI 免疫组织化学 免疫荧光染色 thyroid neoplasms atypical adenoma Ki-67 4＇, 6-diamidino-2-phenylindole immunohistochemistry immunofluores-cence staining
分类号 R736.1 [医药卫生—肿瘤]
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参考文献17

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共引文献4

同被引文献10

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临床与实验病理学杂志
2014年 第10期

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