摘要
目的探讨稳定表达狂犬病毒糖蛋白的重组新城疫病毒疫苗对人肺腺癌A549细胞荷瘤鼠的生长抑制及其可能促凋亡机制。方法将rl-RVG感染到成功建立的A549荷瘤鼠瘤体内,检测对瘤体生长抑制率;RT-PCR检测NDV HN基因及RVG基因的表达;TUNEL检测肿瘤细胞的凋亡;蛋白印迹法测caspase系列、Bax、Bcl-2表达;NDV及PBS作为对照。结果与对照组相比,rl-RVG组荷瘤鼠瘤体增长明显缓慢;RVG及NDV基因表达;rl-RVG组肿瘤细胞凋亡明显增加(P<0.05);rl-RVG组caspase-3,8和9高表达(P<0.01),Bax/Bcl-2比值明显高于对照组(P<0.01)。结论 rl-RVG成功感染至A549荷瘤鼠,其对A549荷瘤鼠瘤体生长有着明显的抑制作用,且可能与rl-RVG激活机体的Bcl-2和caspase家族诱导凋亡相关。
Objective To explore the effects of rl-RVG recombinant adenovirus on the proliferation of A549 lung adenocarcinoma tumor-bearing mice and the mechanism of apoptosis.Methods After being infected A549 lung adenocarcinoma tumor-bearing mice with rl-RVG,the tumor volume was detected,RT-PCR was used to detect the expression of NDV and RVG gene,tunnel assay used to determine the rate of cell apoptosis in tumour paraffin section and Western blot was applied to measure the expression of caspase,Bax and Bcl-2 proteins.Results After rl-RVG infection,the growth of tumor in rl-RVG group was inhibited effectively（P ＜ 0.05） ; RT-PCR showed that RVG gene expressed in rl-RVG group and NDV gene expressed in both rl-RVG and NDV groups.Tunel showed that there were more tumor apoptosis in rl-RVG group （P ＜ 0.05）.Western blot showed that caspase-3,8,9 protein were highly expressed in both rl-RVG and NDV groups（P ＜0.01）.The grayscale ratio of bax/Bcl-2 was higher in rl-RVG group（P ＜0.01）.Conclusions Recombinant rl-RVG successfully,and effectively inhibited the growth of A549 lung adenocarcinoma,which may be attributed to activating Bax and caspase-depended apoptosis pathway.
出处
《基础医学与临床》
CSCD
北大核心
2014年第11期1537-1542,共6页
Basic and Clinical Medicine
基金
江苏省镇江市社会发展项目(SH2013041)
江苏大学医学临床科技发展基金(JLY20120006)
