摘要
为探讨甘薯(Dioscorea esculenta)被薯瘟菌(Ralstonia solanacearum)侵染后的响应,以抗病品种‘湘薯75-55’和感病品种‘胜利百号’为材料,对甘薯叶片的光合特性和叶绿体结构进行了研究。结果表明,薯瘟菌侵染后,‘胜利百号’叶片的细胞膜透性(MP)明显增大,叶绿素(Chl)含量下降,净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)下降,胞间二氧化碳浓度(Ci)和内源ABA含量急剧升高,且细胞膜完整性被破坏,叶绿体数目减少、膨胀模糊、基粒片层松散解体以及淀粉粒变小;而‘湘薯75-55’的各个指标变化不大,细胞膜结构完整,叶绿体基粒片层排列整齐。因此,薯瘟菌侵染后不同抗病品种甘薯叶片在光合特性和叶绿体结构上表现出显著的差异。
In order to understand the response of sweet potato （Dioscorea esculenta） to Ralstonia solanacearum,two varieties, including resistant ‘Xiangshu 75-55’ and sensitive ‘Shenglibaihao’, were used as materials, thechanges in photosynthetic characteristics, abscisic acid （ABA） content and chloroplast ultrastructure were studiedafter infected by R. solanacearum. The results showed that the membrane permeability （MP）, intercellular CO2concentration （Ci） and endogenous ABA content in ‘Shenglibaihao’ infected by R. solanacearum increasedmarkedly, but chlorophyll （Chl） content, net photosynthetic rate （Pn）, transpiration rate （Tr） and stomatalconductance （Gs） obviously decreased. Besides, the integrity of chloroplast membrane system was damaged, thenumber of chloroplast decreased, and expansion to collapse, the grana lamella loosed and disintegrated, and starchgrains got smaller in ‘Shenglibaihao’ leaves affected. In contrast, for ‘Xiangshu 75-55’, the changes in the relatedphysiological indexes were not different significantly, and the plasma membrane and lamellar still kept integration.Therefore, there were distinct differences in the PMP, Chl content, ABA content, Pn and chloroplast structurebetween the two sweet potato varieties with different disease-resistance.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2014年第6期610-616,共7页
Journal of Tropical and Subtropical Botany
基金
福建省自然科学基金项目(2008J04009)
福建省农业科学院原生蔬菜科技创新团队项目(CXTD-2-1320)资助
关键词
甘薯
薯瘟菌
叶片
细胞膜透性
叶绿体
光合特性
Dioscorea esculenta
Ralstonia solanacearum
Leaf
Membrane permeability
Chloroplast
Photosyntheticcharacteristics
