摘要
为探讨大麻根部土壤中是否存在具有脱胶功能的放线菌,采用高氏一号培养基分离纯化大麻根部土壤中的放线菌,利用平板透明圈法筛选具有脱胶功能的菌株,通过形态特征、生理生化特性及分子生物手段鉴定菌种。结果表明:共分离纯化得到41株放线菌,其中菌株DM182具有较强的脱胶能力,其透明圈直径可达3.78 cm,根据菌株DM182的形态、生理生化特性与16S rDNA序列分析将其初步鉴定为白色类诺卡氏菌(Nocardioides albus)。因此,类诺卡氏属(Nocardioides)放线菌具有广阔的应用前景,而白色类诺卡氏菌N.albus在本地区首次筛选得到,具有进一步研究开发的价值。
Whether the degumming strains exist in soil sample collected from the roots of hemp was explored in this study.The actinomycetes were cultivated on GAUZE＇s Medium No.1.The degumming strains of hemp were screened by the method of transparent zones.The strain was classified by physiological,biochemical and morphological characteristics and 16 S rRNA gene sequence analysis.The results showed that a total of 41 actinomyctes were isolated from the samples,of which the strain DM182 was much effective in decomposing the pectin of hemp,and the transparent circle diameter is 3.78 cm.According to its morphological,physiological and biochemical characteristics and 16 S rDNA sequence analysis,the strain DM182 was preliminarily identified as Nocardioides albus.Thus,some members of this genus have a very wide application prospect in agriculture and industry.It is for the first time to report that N.albus strain was isolated in the local region,and the study provided a basis for the further exploration of the strain.
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2014年第11期102-106,共5页
Journal of Textile Research
基金
安徽省高等学校省级自然科学研究项目(KJ2011Z398
KJ2013A266)
六安市定向委托皖西学院市级研究项目(2011LW013
2012LWA019)
皖西学院省级大学生创新训练项目(AH201310376030)
