摘要
分别用绵羊附红细胞体自然感染病羊的全血及分离纯化的绵羊附红细胞体对小白鼠进行攻毒,以建立绵羊附红细胞体人工感染小鼠模型。通过攻毒后症状观察、血液涂片镜检和绵羊附红细胞体特异性PCR检测法,对建立的模型进行评价。结果显示,各试验组小鼠人工感染后3~6 d,血液中均可检测到绵羊附红细胞体,而对照组小鼠未出现异常症状,且血液附红细胞体检查结果为阴性。该研究成功地构建了绵羊附红细胞体小白鼠感染模型,创建的模型可用于附红细胞体的生物学特性、致病机制、药物筛选等方面的研究。
The experimental model of Eperythrozoonsis was established in mice by intravenous and intraperitoneal injection of different doses of whole blood and purified Eperythrozoon ovis from a sheep naturally suffered from Eperythrozoonsis diagnosed by blood pressure drop, blood smear staining and Eperythrozoon ovis specific PCR. The model was further evaluated for its success and characterized. While the mice could be infected within 3-6 days after injection of Eperythrozoon ovis by different means, no obvious infectious symptom was observed in the control groups. The results indicated that the experimental model of Eperythrozoon ovis infection in mice was successfully established and it was potentially helpful for the better understanding of biological property and pathogenic mechanisms of Eperythrozoon ovis.
出处
《畜牧与饲料科学》
2014年第10期1-3,6,共4页
Animal Husbandry and Feed Science
基金
公益性行业(农业)科研专项(201303037-8)
内蒙古农牧业科学院青年创新基金项目