摘要
为了明确河南烟田危害烟草的镰刀菌种类,以烟叶主产区典型病株为样本,用组织分离法获得纯菌株,并对所得菌株进行形态学鉴定、rDNA-ITS序列分析及致病性测定.形态学观察结果表明,分离获得的22个菌株在PDA培养基上均产生茂密的菌丝,呈棉絮状,能产生黄、红、紫等色素,大型分生孢子镰刀形或椭圆形,无色、多胞;小型分生孢子卵形至椭圆形,无色、单胞或双胞.BLAST比对结果表明,在GenBank序列数据库中,菌株1～11号的DNA序列与登陆号为KC429789.1的Fusarium oxysporum （F.oxysporum）序列同源性达到95.77％以上;菌株12～18号与登陆号为JQ910159.1的Fusarium solani （F.solani）序列的同源性达到88.01％以上;菌株19～21号与登陆号HQ176444.1的Fusarium verticillioides （F.verticillioides）序列的同源性达到89.72％以上;菌株22号与登陆号为JX045841.1的Fusarium proliferatum（F.proliferatum）序列的同源性为90.74％.接种试验表明,4种镰刀菌均能侵染活体烟苗并产生典型症状.因此,河南烟田危害烟草的镰刀菌鉴定为F.oxysporum,F.solani,F.verticillioides及F.proliferatum.其中,F.proliferatum侵染烟草在河南为首次报道.
In order to identify the variety of Fusarium causing tobacco diseases in field,the typical infected tobacco plants were sampled from main tobacco growing areas in Henan,pure strains were isolated by tissue separation and identified by morphological method,pathogenicity test,and rDNA-ITS sequences analysis.The results of morphological observation showed that on PDA medium,all of the 22 isolated strains produced dense,gossypine hyphae with yellow,red or purple pigments; macroconidium was oval or sickle in shape,colorless,and multi-cell; microconidium was ovoid to oval in shape,colorless,single-cell or double-cell.BLAST analysis indicated that in GenBank sequence database,the homology of rDNA-ITS sequence was ＞95.77％ between strains No.1-No.11 and Fusarium oxysporum （F.oxysporum,GenBank Accession No.KC429789.1）,which was ＞88.01％ between strains No.12-No.18 and Fusarium solani （F.solani,GenBank Accession No.JQ910159.1）,＞89.72％ between strains No.19-No.21 and Fusarium verticillioides （F.verticillioides,GenBank Accession No.HQ176444.1）,and ＞90.74％ between strain No.22 and Fusarium proliferatum （F.proliferatum,GenBank Accession No.JX045841.1）.Inoculation tests showed that all the mentioned Fusarium could infect living tobacco seedlings and generate typical symptoms.Consequently,the Fusarium infecting tobacco in Henan was identified as F.oxysporum,F.solani,F.verticillioides and F.proliferatum.F.proliferatum infection in Henan tobacco has not been reported before.
出处
《烟草科技》
EI
CAS
北大核心
2014年第11期89-92,共4页
Tobacco Science & Technology
基金
中国烟草总公司科技项目"全国烟草有害生物调查研究"(110200902065)
河南省烟草公司科技项目"河南省烟草有害生物调查研究"(2012M10)
关键词
烟草
镰刀菌
形态鉴定
分子鉴定
致病性
Tobacco
Fusarium
Morphological identification
Molecular identification
Pathogenicity
