摘要
建立了液相色谱-串联质谱法同时测定犬血浆中的替格瑞洛（1）及其主要活性代谢产物AR-C124910XX （2）.使用C18色谱柱,以乙腈∶2 mmol/L乙酸铵溶液（70∶30）为流动相,地西泮为内标,采用TQ-MS检测器,电喷雾离子源（ESI）,以正离子多反应监测（MRM）方式进行定量分析,监测离子对m/z 523.3→m/z 127.1 （1）、m/z 479.3→m/z 153.1 （2）和m/z 284.9→m/z 193.0（内标）.1、2均在50～10 000 ng/ml范围内线性关系良好;提取回收率为109.8％～ 115.1％和109.3％～114.5％,日内RSD≤6.8％,日间RSD≤14.2％.
A LC-MS/MS method was established for the determination ofticagrelor （1） and its active metabolite AR-C124910XX （2） in dog plasma with diazepam as the internal standard.A C18 column was used,with the mobile phase of acetonitrile ∶ 2 mmol/L ammonium acetate solution （70 ∶ 30）.An electrospray ionization source （ESI） was operated in positive ion mode and multiple reaction monitoring （MRM） mode with the transitions of m/z 523.3→m/z 127.1 （1）,m/z 479.3→m/z 153.1 （2） and m/z 284.9→m/z 193.0 （diazepam）,respectively.It was linear for 1 and 2 in the range of 50-10 000 ng/ml.Their extraction recoveries were 109.8％-115.1％ and 109.3％-114.5％,with the intra-day RSDs no more than 6.8 ％ and inter-day RSDs no more than 14.2 ％.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2014年第11期1050-1053,共4页
Chinese Journal of Pharmaceuticals
