摘要
为探讨酶联免疫法（ELISA）检测饲料中沙丁胺醇（salbutamol,SAL）的前处理方法,试验对提取液的pH、离子强度、有机溶剂浓度及样品稀释倍数等条件进行了考察,选取最适宜的提取液条件对2种不同类型的饲料样本进行提取,用ELISA测定样品中沙丁胺醇的残留量。结果表明,沙丁胺醇标准曲线的IC50值为0.606ng/mL,线性范围为0.221～1.658ng/mL,R^2=0.9998,提取液的pH为7.5,PBS缓冲液最优浓度为0.06mol/L,稀释倍数为10倍,2种不同类型的饲料样本的检测限（LOD）为5.0ng/mL。当沙丁胺醇的添加浓度为5、10μg/kg时,该方法的添加回收率为77%～110%,变异系数〈8%。
Objective of the study was to develop a preparation treatment for salbutamol in feed by enzyme-linked immunosorbent assay （ELISA）. The assay optimized the influence factor of the preparation and used to extract salbutamol residues in feed. The results showed that the inhibition concentration （IC50） for the developed ELISA was 0. 606 ng/mL with the detection range of 0. 221 to 1. 658 ng/mL and R^2 was 0. 9998. After optimization,pH 7.5,0.06 mol/L PBS with the dilution ratio of 10 was selected to extract salbutamol residues in feed and the limit of detection （LOD） was 5. 0 ng/mL in feed. The recoveries ranged from 77% to 110% for the spiked samples （5 and 10 μg/kg） ,and RSD was less than 8%.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第11期179-183,共5页
China Animal Husbandry & Veterinary Medicine
基金
公益性行业(农业)科研专项(2012104003-5)
关键词
酶联免疫法
沙丁胺醇
前处理
饲料
enzyme-linked immunosorbent assay （ELISA）
salbutamol
preparation treatment
feed
