摘要
目的探讨1,25-二羟维生素D3(1,25(OH)2D3)对IgA肾病大鼠Th1细胞(IFN-γ)的影响。方法将造模成功的32只IgA肾病模型大鼠随机分为强的松治疗组、1,25(OH)2D3治疗组、强的松+1,25(OH)2D3联合治疗组及未干预组,同时设立正常对照组。检测各组大鼠24h尿蛋白、血肌酐及IFN-γ的水平。结果 1A组(正常对照组)、B组(未干预组)、C组(强的松治疗组)、D组(1,25(0H)2D3治疗组)及E组(强的松+1,25(0H)2D3联合治疗组)的24h尿蛋白分别是7.03±0.99mg/d、51.49±3.04mg/d、12.15±0.75mg/d、23.0±2.27mg/d及9.99±0.79mg/d,其中A组、C组、D组及E组的尿蛋白水平较B组下降,差别有统计学意义(F=47.778,P<0.05)。2A组、B组、C组、D组及E组的脾脏IFN-γ水平分别是0.64%±0.05%、3.63%±0.22%、2.11%±0.13%、1.33%±0.10%及0.97%±0.17%,相应的淋巴结IFN-γ水平分别是2.13%±0.42%、17.32%±0.65%、8.44%±0.38%、6.39%±0.57%及3.01%±0.27%,其中A组、C组、D组及E组的IFN-γ水平较B组显著下降,差别有统计学意义(F=479.381,P<0.05)。结论 1,25(OH)2D3可降低IgA肾病大鼠尿蛋白及IFN-γ的水平。
Objective Explore the mechanism of 1α,25-dihydroxyvitaminD3 regulation on Th1 cells in IgA nephropathy rats.Method The model of 32 IgA nephropathy rats were randomly divided into prednisone treatment group,1α,25-dihydroxyvitaminD3 treatment group,prednisone ＋ 1α,25-dihydroxyvitaminD3 treatment group and non treatment group.At the same time,we set up the normal control group.24 hours urine protein,serum creatinine value and IFN-γwere measured in each group.Results ①The level of 24-hour urine protein in group A,B,C,D and E,are respectively 7.03±0.99mg/d,51.49±3.04mg/d,12.15±0.75mg/d,23.0± 2.27mg/d and 9.99±0.79mg/d,in which the group A,C,D and E decreased significantly compared with the group B （F=47.778,P＜0.05）.②The level of IFN-γin spleen of group A,B,C,D and E are respectively 0.64％±0.05％,3.63％±0.22％,2.11％±0.13％,1.33％±0.10％ and 0.97％±0.17％.The level of IFN-γin Lymph Node of group A,B,C,D and E are respectively 2.13％ ± 0.42％,17.32％ ± 0.65％,8.44％ ± 0.38％,6.39％ ± 0.57％ and 3.01％±0.27％,among the group A,C,D and E decreased significantly compared with the group B（F=479.381,P＜0.05）.Conclusion 1α,25-dihydroxyvitaminD3 can reduce the level of urinary protein and IFN-γin IgA nephropathy rats.
出处
《中国血液净化》
2014年第10期722-724,共3页
Chinese Journal of Blood Purification
基金
山西省国际科技合作项目(2013081056)
山西省回国留学人员科研资助项目(2013-117)
太原市科技资助项目(12016904)
山西省科技攻关项目(20130313022-3)
国家自然基金(81102198)
