摘要
目的探讨自身免疫性肝病(AILD)抗体谱检测在AILD诊断中的临床应用价值。方法采用线性免疫分析法检测不明病因肝功能异常、各种病毒性肝炎以及健康体检者(健康对照组)的血清标本,进行抗线粒体亚型-丙酮酸脱氢酶复合物(AMA/M2)、抗肝肾微粒体Ⅰ型抗体(LKM-1)、抗肝细胞胞浆Ⅰ型抗体(LC-1)、抗可溶性肝抗原/肝胰抗原抗体(SLA/LP)、糖蛋白210抗体(gp210)以及多核点抗原抗体(sp100)等6项指标的检验结果资料进行回顾分析。结果各组患者标本中的自身抗体总阳性检出率分别为自身免疫性肝炎(AIH)80.5%、原发性胆汁性肝硬化(PBC)92.3%、乙型肝炎病毒(HBV)17.2%、丙型肝炎病毒(HCV)16.7%、甲型肝炎病毒(HAV)22.2%、戊型肝炎病毒(HEV)14.3%,对照组仅为2.6%;AILD组的阳性检出率与其他各组间比较(P<0.05);AIH各亚型中AIH-Ⅰ的AMA/M2的阳性检出率与AIH-Ⅱ和AIH-Ⅲ比较(P<0.05),AIH-Ⅱ的LKM-1的阳性检出率与AIH-Ⅰ和AIH-Ⅲ比较(P<0.05),AIH-Ⅲ的SLA/LP的阳性检出率与AIH-Ⅰ和AIH-Ⅱ比较(P<0.05)。结论 AILD抗体谱检测有助于AILD的早期诊断,可为该病的分型以及治疗提供有价值的实验室依据。
Objective To investigate the clinical application value of the autoimmune liver disease antibody spectrum detection in diagnosing autoimmune liver diseases（AILD）.Methods The linear immunoassay was adopted to detect the serum specimens in the patients with unknown etiology of liver function abnormalities,various viral hepatitis and healthy subjects with physical examination（control group）.The detection results of 6indexes of anti-mitochondrial subtype-pyruvate dehydrogenase complex（AMA/M2）,liver kidney microsomal antibody type 1（LKM-1type）,anti-liver cell cytoplasmic antibody-1（LC-1）,type I anti soluble liver antigen/liver pancreas antibody（SLA/LP）,glycoprotein 210antibody（gp210）and multi nucleus antigen antibody（Sp100）were retrospectively analyzed.Results The total positive detection rate of autoantibodies in various groups were 80.5%in autoimmune hapatitis（AIH）,92.3%in primary biliary cirrhosis（PBC）,17.2%in hepatitis B virus（HBV）,16.7%in hepatitis C virus（HCV）,22.2%in hepatitis A virus（HAV）and 14.3%in hepatitis E virus（HEV）,while which in the control group was only 2.6%;the positive detection rate in the autoimmune diseases group had statistical difference compared with other groups（P〈0.05）;in the various AIH subtypes,the positive detection rate of AMA/M2 had statistical difference between AIH-Ⅰwith AIH-Ⅱand AIH-Ⅲ（P〈0.05）,the positive detection rate of LKM-1had statistical difference between AIH-Ⅱwith AIH-Ⅰand AIH-Ⅲ（P〈0.05）,the positive testing rate of SLA/LP had statistical difference between AIH-Ⅲ with AIH-Ⅰand AIH-Ⅱ（P〈0.05）.Conclusion The AILD antibody spectrum detection conduces to early diagnosis of AILD and can provide the valuable laboratory basis for typing this disease and treatment.
出处
《检验医学与临床》
CAS
2014年第21期2998-3000,共3页
Laboratory Medicine and Clinic
