摘要
目的:调查闽东地区汉族与畲族人群CYP2C19基因多态性分布情况。方法:采用限制性片段长度多态性聚合酶链反应(PCR-RFLP),对汉族(n=140)与畲族(n=180)人群CYP2C19两个多态性位点CYP2C19*2和CYP2C19*3的基因多态性进行分析,用SPSS16.0软件进行统计分析,Hardy-Weinberg遗传平衡定律验证基因型分布情况。结果:汉族与畲族人群各种基因型出现频率分别为:*1/*1 14.3%,28.9%;*1/*2 28.6%,30.0%;*1/*3 11.4%,16.7%;*2/*2 21.4%,9.4%;*2/*3 21.4%,12.8%;*3/*3 2.9%,2.2%。汉族与畲族CYP2C19不同等位基因出现频率:*1为34.3%,52.2%;*2为46.4%,30.8%;*3为19.3%,16.9%。结论:闽东地区畲族与汉族CYP2C19*2与*3等位基因突变率存在差异,畲族人群基因突变率低于汉族(P<0.05)。
Objective： To investigate the distribution of CYP2C19 gene polymorphism of the Han and the She nationalities in eastern Fujian Province. Methods Detection of cYPgC19 · 2 and CYP2C19·3 mutant alleles of the Han（n=140） and the She（n= 180） nationalities was performed using polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed by SPSS16.0 software. CYP2C19 genotype frequencies of the Han and the She nationalities in eastern Fujian Province were verified by Hardy-Weinberg equilibrium. Resuits：CYP2C19 genotype frequencies of the Han and the She nationalitieswere·l/·1 14.3%,28.9%· 1/· 2 28. 6%,30.0·/0· 1/· 3 11. 4%,16. 7%· 2/· 2 21. 4%,9. 4%;· 2/· 3 21.4%, 12.8% ; · 3/· 3 2.9% ,2.2% ,respectively. The mutant alleles frequencies of CYP2C19 of the Han and the She national- ities were, · 1 34.3%,52.2%· 2 46.4%,30.8%;· 3 19.3%,16.9% ,respectively. Conclusion：The mutant alleles frequencies of CYP2C19· 2 and CYP2C19· 3 of the She nationality were significantly lower than those of the Han nationality（P〈0.05）.
出处
《药学服务与研究》
CAS
CSCD
2014年第5期364-367,共4页
Pharmaceutical Care and Research
基金
福建省宁德市科技计划项目(20120070)
