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腺病毒介导的IL-24增强紫杉醇对A549肺腺癌细胞生长的抑制作用 被引量：3

Adenovirus-mediated interleukin-24 enhances the inhibitory effect of paclitaxel on the growth of lung cancer A549 cells
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摘要 目的探讨腺病毒介导的白细胞介素24(Ad-IL-24)联合紫杉醇对体外培养的人肺腺癌A549细胞生长的影响。方法用Ad-IL-24、紫杉醇、Ad-IL-24联合紫杉醇处理A549细胞,以CCK-8法测定细胞生长抑制率,流式细胞术检测细胞凋亡和周期的变化。结果 Ad-IL-24可明显抑制人肺腺癌A549细胞生长并诱导细胞凋亡,与紫杉醇联合处理后,其抑制肺癌细胞生长及诱导细胞凋亡作用明显优于单用紫杉醇组及Ad-IL-24组,并引起G2/M期阻滞。结论 Ad-IL-24感染肺癌A549细胞可增强紫杉醇对肿瘤细胞生长的抑制作用。 Objective To investigate the effect of adenovirus-mediated interleukin-24（ Ad-IL-24） combined with paclitaxel on the growth of human lung cancer A549 cells in vitro. Methods A549 cells were treated with Ad-IL-24 alone,paclitaxel alone and Ad-IL-24 combined with paclitaxel,respectively. Their proliferation was detected by CCK-8 assay,and the changes of apoptosis and cell cycle were tested by flow cytometry. Results The treatment of Ad-IL-24 significantly inhibited the A549 cell growth and induced cell apoptosis. Compared with paclitaxel alone and Ad-IL-24 alone,the Ad-IL-24 plus paclitaxel treatment more evidently inhibited lung cancer cell growth and increased cell apoptosis rate,and induced G2 /M phase arrest.Conclusion Ad-IL-24 infection can enhance the inhibitory effect of paclitaxel on lung cancer cell growth.
作者 徐明菊 李敏敏 杨吉成 郭锦锦 孙万邦
机构地区 遵义医学院珠海校区 苏州大学医学部细胞与分子生物学教研室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2014年第11期1150-1153,1158,共5页 Chinese Journal of Cellular and Molecular Immunology
基金 贵州省免疫学研究生教育创新基地重点培育项目(201101) 遵义医学院研究生学术创新课题(CX201321)
关键词 重组腺病毒 白细胞介素24 紫杉醇 肺癌细胞 细胞生长 adenovirus IL-24 paclitaxel lung cancer cell cell growth
分类号 R392.11 [医药卫生—免疫学] R734.2 [医药卫生—肿瘤]
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参考文献18

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二级参考文献37

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共引文献8

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二级引证文献22

细胞与分子免疫学杂志
2014年 第11期

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