摘要
目的探讨姜黄素(curcumin)对猪骨髓间充质干细胞(BMSCs)增殖和成脂分化的影响及机制。方法用含不同浓度姜黄素的细胞培养液和诱导分化液,培养和诱导分化猪BMSCs,用MTT比色法检测对BMSCs增殖的影响,用形态学和油红O染色提取法检测对成脂分化的影响,用实时荧光定量PCR(Real-time PCR)检测对成脂分化相关基因过氧化物酶体增殖物激活受体γ(PPARγ2)和脂蛋白脂肪酶(LPL)mRNA表达的影响。结果当姜黄素用于猪BMSCs的培养,1μmol/L浓度在培养第3天、第5天和10μmol/L浓度在培养第5天开始能够显著促进细胞增殖(P<0.05),但高于15μmol/L则具有显著的抑制作用(P<0.05);当姜黄素用于猪BMSCs的成脂诱导分化,所用不同浓度的姜黄素都能显著抑制成脂分化(P<0.05),用0.5μmol/L获得28.3%的抑制率,而用20μmol/L获得71.24%的高抑制率;当用20μmol/L姜黄素处理和分别诱导分化5、10和15d,PPARγ2 mRNA表达的抑制率分别达到19.11%、49.52%和76.76%,LPL mRNA表达的抑制率分别达到37.58%、49.32%和74.70%。结论适当浓度姜黄素具有促进猪BMSCs增殖和抑制猪BMSCs向脂肪细胞分化的作用,对脂肪细胞的分化发挥了重要调控作用。
Objective To investigate the effects and mechanisms of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells （BMSCs）. Methods The porcine BMSCs were cultured and differentiated with cell culture and adipegenic differentiation medium containing different concentrations of curcumin, the effects of curcumin on proliferation of porcine BMSCs were detected by MTT, the effects of curcumin on adipogenic differentiation were analyzed by morphology and oil red O, and the expressions of peroxisome proliferator-activated receptor γ（PPARγ2） mRNA and lipoprotein lipase（LPL） mRNA were detected by Real-time PCR. Results When the porcine BMSCs were cultured with curcumin, 1 μmol/L curcumin at the third days of culture and 5, 10μmol/L cureumin at the fifth days of culture significantly promoted cell proliferation （ P 〈 0. 05 ） , but curcumin concentration higher than 15μmol/L inhibited cell proliferation （P 〈 0. 05 ）. When the porcine BMSCs were differentiated with curcumin, the different concentrations of curcumin significantly inhibited adipogenic differentiation （ P 〈 0. 05 ）. The inhibition rate of 0. 5 Ixmol/L curcumin was 28.3% and 20μmol/L curcumin was 71.24%. When the cells were treated with 20μmol/L curcumin and differentiated in 5, 10 and 15 days, the inhibition rates of expressions of PPARγ2 and LPL mRNA were 19.11%, 49.52% , 76.76% and 37.58% , 49.32% , 74.70% respectively. Conclusion The proper concentrations of curcumin were able to promote the proliferation and inhibit adipogenic differentiation of porcine BMSCs, suggesting that curcumin may play an important role in the regulation of adipocyte differentiation.
出处
《解剖学报》
CAS
CSCD
北大核心
2014年第6期793-799,共7页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(31372392)
