摘要
目的:建立二乙基亚硝胺(diethylmitrosamine,DEN)诱导仓鼠肝癌模型的方法.方法:取体质量为70-100 g的叙利亚仓鼠24只,随机分为实验组(n=12)和对照组(n=12),造模组采用腹腔注射与饮用DEN水溶液诱发仓鼠肝癌,对照组给予等量生理盐水腹腔注射及正常饮水,常规病理HE染色检查.结果:病理检查证实仓鼠肝癌为肝细胞癌,肝癌发生率为66.7%(8/12).裸鼠成瘤率为62.5%(5/8).结论:DEN可成功诱导仓鼠肝癌,方法简单,诱癌成功率高.同时该模型能够较好地模拟人类肝癌发展的过程,可作为动态研究肝癌发生的新动物模型.
AIM： To establish a hamster model of diethylnitrosamine（DEN） induced hepatocellular carcinoma. METHODS： Twenty-four hamsters, weighing from 70 to 100 g, were randomly divided into either an experimental group（n = 12） or a control group（n = 12）. The experimental group was injected with DEN via the abdominal cavity and simultaneously administered DEN in drinking water. In the control group,DEN was replaced with normal saline. Pathological morphology was observed after HE staining. RESULTS： Pathology results confirmed that hepatocellular carcinoma was successfully induced in 8 hamsters of the experimental group, and the incidence rate of liver cancer was 66.7%（8/12）. The rate of xenograft tumor formation in nude mice was 62.5%（5/8）. CONCLUSION： A hamster model of DEN induced hepatocellular carcinoma has been established successfully, and the incidence of hepatocellular carcinoma is high. This model can be used as a new and dynamic animal model.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第31期4795-4799,共5页
World Chinese Journal of Digestology
基金
国家自然基金资助项目
No.81260354~~
关键词
二乙基亚硝胺
肝癌
动物模型
Diethylmitrosamine
hepatocellular carcinoma
Animal model
