摘要
菲利普孢囊线虫(Heterodera filipjevi)是我国黄淮麦区新发现的一种病原线虫,但在小麦属中缺乏有效抗源。为从小麦近缘种属中发掘抗病资源,采用室内接种平均单株雌虫鉴定法和相对抗病指数法,从34份卵穗山羊草(Aegilops geniculata Roth)材料中筛选出6份抗H.filipjevi的种质材料,其中PI542187表现高抗,PI564186、PI573396、PI374365、PI361880和PI374365表现抗病。对中国春–卵穗山羊草染色体附加系抗性鉴定,发现卵穗山羊草7U g和5M g附加系的单株白雌虫数明显低于中国春。连续两年又对17份小麦–卵穗山羊草5M g-5D易位系材料进行抗性鉴定现发,5M g464、5M g466和5M g457抗性较好。
Heterodera filipjevi is a pathogenic nematode of wheat newly discovered in Huang-Huai Plain of China. However, germplasm resistant to H. filipjevi has been rarely found in genus Triticum. To mine resistance genes from wheat relative species, we identified the resistance to H. filipjevi in 34 accessions of Aegilops geniculata by inoculating H. filipjevi Xuchang population with the mean female number per plant method and relative resistance index methods in greenhouse. Six candidates of germplasm were identified including PI542187 in high resistance and other five accessions (PI564186, PI573396, PI374365, PI361880, and PI374365) in resistance. A set of Chinese Spring (CS)–Ae. geniculata addition lines were used to chromosomally locate the resistance gene(s). Lines 7Ug and 5Mg showed obviously fewer females per plant than CS. In two consecutive years, 17 CS–Ae. geniculata 5Mg-5D translocation lines were further tested for resistance against H. filipjevi. Lines 5Mg464, 5Mg466, and 5Mg457 exhibited resistance to H. filipjevi.
出处
《作物学报》
CAS
CSCD
北大核心
2014年第11期1956-1963,共8页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)项目(2012AA101105)
河南省农业科技创新项目(2012-04)资助
关键词
卵穗山羊草
菲利普孢囊线虫
附加系
易位系
抗性鉴定
Aegilops geniculata
Heterodera filipjevi
Additional line
Translocation line
Resistance identification