摘要
目的:探讨NF-кB在肝癌介入治疗后肿瘤组织和癌旁肝细胞中的表达及其变化特点。方法:建立VX2肝癌模型兔59只,分为A组(肝癌组)29只,B组(肝癌经导管肝动脉栓塞介入组)30只、C组(对照组)20只,免疫组化检测各组肝癌及癌旁肝组织中的NF-кB表达。结果:A组肝癌组织与癌旁肝组织NF-κB阳性表达率分别为72.4%(21/29)、37.9%(11/29),肝癌介入治疗后肝癌组织和癌旁组织NF-κB阳性表达率82.4%(24/30)、60%(18/30),C组NF-κB阳性表达率为10%(2/20),各组与对照比较,除A组癌旁肝组织外差异均有统计学意义(P<0.05),其余两两比较差异无统计学意义(P>0.05)。结论:治疗前癌旁组织,其内NF-кB的表达水平尚处于较低水平,介入治疗后的癌旁肝组织的脂肪变性及炎性反应等因素可能会导致NF-κB的表达增加。
Objective： To explore the expression of NF-кB in Liver carcinoma tissues and adjacent noncancerous liver tissue post interventional therapy and its characteristic changes. Methods： 59 rabbits with VX2 carcinoma model were randomly divided into 2 groups, 29 in group A （liver cancer）, and 30 in group B （ liver cancer/transcatheter hepatic arterial embolization, TACE） as a study group. And in group C 20 rabbits with no liver cancer as a control group. Immunohistochemical detection of NF-кB in liver cancer tissue and normal tissues adjacent to liver cancer tissue was done. Results：In group A, positive expression rate of NF-κB was 72.4%（21/29） in cancerous liver tissue and 37.9 %（ 11/29） in liver tissue adjacent to carcinoma. In group B （post interventional therapy） positive expression rate of NF-κB was 82.4%（24/30） in liver cancer tissues and 60%（18/30） in adjacent tissues. In group C, NF-κB positive expression rate was 10%（2/20）. Then each group was compared with control. Except in liver tissue adjacent to carcinoma in group A, the data recorded was statistically significant （P 〈 0.05）. The other two data had no statistical significance （P 〉 0.05）. Conclusion： The expression level of NF-κB is relatively low before interventional therapy in the tissue adjacent to carcinoma. Post interventional therapy. There is increased expression of NF-κB, may be due to the fatty degeneration of liver tissues adjacent to carcinoma and inflammatory reaction.
出处
《大理学院学报（综合版）》
CAS
2014年第10期42-46,共5页
Journal of Dali University
基金
国家自然科学基金资助项目(81241131)
云南省教育厅科学研究基金资助项目(2013J113)
关键词
NF-кB
肝癌
介入治疗
NF-кB
liver carcinoma
interventional therapy
