摘要
目的:利用本实验室制备的嗜水气单胞菌灭活疫苗免疫银鲫,研究疫苗的免疫保护效果。方法:将银鲫随机分为3组:注射组、浸泡组和对照组进行试验,二次免疫后,分别对银鲫组织和血液样品进行组织病理学、血液免疫学研究以及免疫保护率的测定。结果:血液指标检测显示,注射组、浸泡组各项血液指标发生显著改变,银鲫各项免疫指标显著提高;组织病理学显示,攻毒后,对照组的肝、肾、脾等重要组织已发生病变,而注射组和浸泡组无明显的变化;注射组、浸泡组和对照组的免疫保护率分别为90%、60%、0%。结论:嗜水气单胞菌灭活疫苗对银鲫的细菌性出血病具有显著的保护作用。
Objective： In order to find a safe and healthy way to control fish diseases caused by Aeromonas hydrophila,the immune effect of Aeromonas hydrophila inactivated vaccine on Carassius gibelio was studied. Methods： Three groups were randomly made by each of 10 fishes. Injection groups was made intraperitoneally（ i. p.） with 200 μl,and soaked group with 3 L volumes of inactivated vaccine suspensions respectively while the control group with same volumes of saline. Tissues and blood samples of carassius gibelio were collected after the second vaccination. Then the histopathological,immunological indicators and survival percentage were detected. Results： The relative survival percentage of two groups was 90% and 60% respectively. The serum enzymes of injection and soaked group showed significant differences contrasted with the control group. Histopathological studies revealed that the important tissues have been damaged obviously after the bacterium challenge,while the experiment groups were no damage. Conclusion： Therefore the inactivated vaccine could prevent carassius gibelio diseases that caused by A. hydrophila.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第11期1499-1503,1507,共6页
Chinese Journal of Immunology
基金
河南省科技厅科技攻关(132102310165)
河南省教育厅科技攻关(2011A240001)项目
关键词
嗜水气单胞菌
组织病理切片
灭活疫苗
免疫学指标
免疫保护率
Aeromonas hydrophila
Tissue section
Inactivated vaccine
Immunological indicators
Protection rate
