摘要
脾肾阳虚泄泻是中医常见病症。迄今为止,还没有理想的脾肾阳虚泄泻大鼠模型可供研究。该文通过比较单因素(造模方法Ⅰ,注射氢化可的松+灌胃番泻叶)和复合因素(造模方法Ⅱ,灌胃腺嘌呤+饮食失节+劳倦过度+灌胃番泻叶)2种造模方法,以期建立符合中医证候的脾肾阳虚泄泻大鼠模型。该研究选取大鼠的腹泻指数、尿D-木糖排泄率、NOS/c GMP信号传导系统、脏器指数和病理组织学检查等指标,分别对2个模型组大鼠进行比较评价。结果表明,与正常组相比,造模Ⅰ组、Ⅱ组大鼠的所有考察指标均有显著性差异(P<0.01,P<0.05),且造模Ⅱ组在腹泻指数、NOS/c GMP信号通路、肾脏、睾丸和胸腺的脏器指数及病理组织检查等指标表征上与造模Ⅰ组相比有显著性差异(P<0.01,P<0.05)。综合以上研究,以腺嘌呤致肾阳虚,饮食失节、劳倦过度同灌胃番泻叶致脾阳虚复合因素的造模方法,更符合脾肾阳虚泄泻模型的症候。因此该研究为在此动物模型基础上研究温肾暖脾,涩肠止泻方药的作用提供基础。
Spleen kidney Yang deficiency （SKYD） diarrhea is a common syndrome in tranditional Chinese medicine （TCM）. Until now, there is not an ideal SKYD diarrhea rat model for the research. In this study, we compared single factor way （ method I ,injecting hydrocortisone and gavaging Sennae Folium） with compound factors way（method II, garaging adenine, improper diet, exhaus- tion, and gavaging Sennae Folium） on establishing SKYD diarrhea rat model. After modelling, diarrhea index, D-xylose excretory rate, NOS/cGMP signal transduction system, organ index and histopathology examination were used to evaluate the two ways. The results showed that, compared with health group, all the assessment criterias of method I and method I1 had significant differences （P 〈 0.01, 0.05 ）. In addition, the index such as diarrhea index, NOS/cGMP signal transduction system, organ index （ kidney, testis and thymus） and histopathology examination had significant differences（P 〈0.01,0. 05） between method I and method I1. In conclusion, the compound factors modelling method better conforms to the symptom of diarrhoea model caused by SKYD. This new modelling method provides a basisfor studying on TCM astringents warming and tonifying the spleen and kidney, relieving diarrhea.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第23期4658-4663,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81274085)
关键词
脾肾阳虚
泄泻大鼠模型
单因素
复合因素
spleen kidney Yang deficiency
diarrhea rats model
single factor
compound fator
