摘要
目的:探讨在低氧条件下,经腺苷受体A2b(A2b AR)拮抗剂PSB603干预后少突胶质前体细胞(OPC)的改变。方法:原代培养新生SD大鼠脑皮层OPC,随机分为对照组、低氧组、PSB603组及实验组,实验组在2%低氧条件下再给予PSB603处理,4 d后用倒置相差显微镜观察各组细胞的形态学差异,并用MTS检测各组细胞吸光值的差异。结果:与对照组比较,PSB603组的吸光值和形态均无明显差异,而低氧组的吸光值明显升高,且具有多分支或网状的细胞明显增多;与低氧组比较,实验组的吸光值明显下降(P<0.01),且具有多分支或网状的细胞明显减少。结论:低氧能够加速OPC的分化,PSB603能部分阻断低氧所诱导OPC的分化,提示A2b AR在低氧所诱导的OPC分化过程中发挥着重要的作用。这将对治疗脱髓鞘相关疾病以及进一步认识A2b AR在神经系统疾病中的作用具有重要意义。
Objective：To investigate the variation of oligodendrocyte precursor cells（ OPCs） with PSB603 intervention,the antagonist of adenosine receptor A2b（ A2 b AR）,exposed to 2% hypoxia.Methods：All the OPCs cultured from neonatal SD rat cortex were randomly divided into control group,hypoxia group,PSB603 group and experiment group which were treated by PSB603 under the condition of 2% oxygen.Four days later the differences among the groups were detected by inverted phase contrast microscope and MTS absorbance values.Results：Compared with control group,the OD values and morphology of PSB603 group were not significantly different,the OD value of hypoxic group was significantly increased and the number of cells with multiple branches or reticular branches increased significantly;compared with hypoxic group,both OD value and number of cells with multiple branches or reticular branches of experiment group relatively decreased（ P〈0.01）.Conclusion：Hypoxia can accelerate OPC differentiation,which can partly be inhibited by PSB603.This suggests that the A2 b AR play an important role in the OPC differentiation process induced by hypoxia.This will be meaningful to treatment of demyelinating diseases and further understanding the role of adenosine receptor A2 b AR in the central nervous system diseases.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第6期661-665,共5页
Chinese Journal of Neuroanatomy
基金
宁夏自然科学基金(NZ12189)
