摘要
目的优化空肠弯曲菌(CJS131)诱导系统性红斑狼疮(SLE)样小鼠模型的方法。方法小鼠随机分为5组,即正常对照组、卡介苗(BCG)对照组、结核分枝杆菌H37Ra对照组、CJS131+BCG模型组和CJS131+H37Ra模型组。在第0天免疫,在第14、21、42天加强免疫,在第36、54、61天分批称其体质量后处死。检测小鼠免疫器官指数、血清抗核抗体水平、血清总Ig G水平和肾组织病理损伤程度。结果不论采用含BCG还是H37Ra的弗氏完全佐剂(FCA),给予CJS131免疫的小鼠均有SLE样综合征的表现:血清抗核抗体及总Ig G水平升高,肾组织损伤。在动态检测中病变基本维持。结论 CJS131在对模型组小鼠的免疫诱导中起主要作用,以BCG或H37Ra作为FCA中采用的菌株都可引起病变,通过加强免疫可维持小鼠长期病变,优化后的模型可用于SLE治疗性药物的筛选。
AIM To optimize a mouse model of systemic lupus erythematosus （ SLE ） induced by Carapylobacter jejuni-S131 （CJS131）. METHODS The mice were divided into 5 groups randomly： normal control, BCG （Bacille Cal- mette-Gu^rin） control, H37Ra （Mycobacterium tuberculosis H37Ra ） control, CJS131 ＋ BCG model and CJS131 ＋ H37Ra model. Mice were immunized on day 0 and boosted on day 14, day 21, day 42 and then be sacrificed on day 36, day 54, day 61 in turn. The levels of organ index, serum anti-nuclear antibodies, serum total IgG and the degree of patho- logical lesions of kidney were evaluated. RESULTS The mice immunized by CJSI31 and FCA（Freund＇s complete adju- vant） （no matter with BCG or H37Ra ） had SLE like syndromes： the levels of serum anti-nuclear antibodies and total IgG of model groups were higher than those of the control groups. The kidneys of model groups had pathological lesions. All the pathological changes persisted in the dynamic detection. CONCLUSION CJSI31 plays a major role inducing lupus in mice. Model groups immunized by CJSI31 plus FCA both have pathological injury, no matter with BCG or H37Ra in FCA. The pathological syndromes in boosted mice can persist for a long time. This study can be used in screening of therapeutic medicines for SLE.
出处
《中国临床药学杂志》
CAS
2014年第6期343-348,共6页
Chinese Journal of Clinical Pharmacy
基金
国家自然科学基金(编号81274165)
上海市科委基础研究重点项目(编号12JC1400800)
