摘要
在对大豆、烟草与地黄寄主上14个大豆孢囊线虫(SCN)种群形态与分子鉴定的基础上,对雌虫的孢囊和阴门锥进行形态观察和测计。结果表明:烟草SCN和大豆SCN的各测量值之间无明显差异(P>0.05),地黄SCN的阴门窗长(43.8μm)和宽(33.9μm)显著小于其他两者(P<0.05);同一寄主不同SCN种群之间的形态也存在一定差异;阴门裂长和下桥长具有稳定性,可以作为鉴定大豆孢囊线虫可靠的形态特征。3种寄主种群侵染大豆(‘荷豆12号’)的试验表明,大豆SCN能侵染大豆并能形成雌虫,而烟草SCN和地黄SCN未能侵染大豆和形成雌虫。本研究结果表明SCN不同寄主种群存在形态变异,而它们对大豆的寄主适应性也不相同。
Based on both morphological and molecular identification of 14 soybean cyst nematode （SCN）popula-tions on soybean,tobacco and rehmannia,cyst and vulval cone were characterized by morphological observation and morphometry. The results showed that SCN populations collected from tobacco and soybean had no obvious difference in morphological measurements （P＆gt;0.05）,but the fenestral length （43.8μm）and width （33.9μm）in the population collected from rehmannia were obviously shorter than those of SCN from other two hosts （P＆lt;0.05）. There were some morphological differences in different SCN populations collected from the same host. The length of vulval slit and underbridge could be used as reliable identification characters for their high stability.SCN populations collected from soybean could infect soybean cultivar‘Hedou 12’and produced females;however,the popula-tions collected from tobacco and rehmannia failed to infect the soybean cultivar or formed females. The results indicated that there were variations in morphological characters and host adaptability of different populations of SCN.
出处
《植物保护》
CAS
CSCD
北大核心
2014年第6期106-111,共6页
Plant Protection
基金
中国烟草总公司科技项目(110200902065)
公益性行业(农业)科研专项(200903040)
山东省“泰山学者”建设工程专项
关键词
大豆孢囊线虫
形态变异
分子鉴定
寄主适应性
Heterodera glycines
morphological variation
molecular identification
host adaptability
