摘要
目的探讨绝经后老年女性阴道微生态的变化。方法选取来我院体检中心体检、无自觉症状且阴道微生态正常的50例绝经后老年女性和68例育龄期女性，通过聚合酶链反应法（PCR）检测绝经后老年女性阴道内乳杆菌的种类和数量，并分析其与育龄期女性的差异。结果在绝经后女性中，92％（46例）阴道中仅有1种乳杆菌菌种存在，8％（4例）的女性有2种乳杆菌；而仅4％（3例）的育龄期女性阴道中只有1种乳杆菌菌种，96％（65例）的育龄期女性阴道中同时有2种及以上的菌种并存，育龄期女性阴道乳杆菌种类较绝经后女性更多（χ^2=91．035，P=0．000）。绝经后女性阴道乳杆菌按检出率高低排序前4位依次为詹氏乳杆菌（30．0％）、加氏乳杆菌（22．6％）、卷曲乳杆菌（16．9％）和惰性乳杆菌（13．2％）；育龄期依次为惰性乳杆菌（80．8％）、卷曲乳杆菌（70．5％）、加氏乳杆菌（67．6％）和詹氏乳杆菌（39．7％）。实时PCR检测女性阴道乳杆菌16srDNA的含量，育龄期女性阴道内总乳杆菌16srDNA含量均数较绝经后女性多[（87．3±0．8）ng／μg总DNA比（35．1±3．6）ng／μg总DNA，t=6．514，P=0．0483结论绝经后老年女性阴道内定植的乳杆菌种类与育龄期妇女不同，数量明显减少。
Objective To investigate the changes and significances of vaginal microecology in elderly postmenopausal women. Methods 50 postmenopausal women and 68 reproductive-age women with no subjective symptoms and normal vaginal microeeological who had health examination in Beijing Hospital were selected. The contents and strains of vaginal lactobacilli in both groups were detected by polymerase chain reaction（PCR）, and their differences between the two groups were analyzed. Results 92% （46 cases） of postmenopausal women had only one vaginal lactobaeillus strain in vaginal, and 8% （4 cases） of them had two lactobacillus, strains in vaginal. But 4 % （3 cases） of reproductive-age women had only one vaginal laetobacillus strain in vaginal, and 96% （65 cases） of them had two and more lactobacillus strains in vaginal. There was a significant difference in the strains of vaginal laetobacilli between the two groups （χ^2 = 91. 035, P= 0. 000）. The strains of vaginal lactobaeilli ranking top 4 detection rate was Laetobacillus jensenii （30.0%）, Lactobacillus gasseri （22.6%）, Lactobaeillus crispatus （16.9%） and Lactobaeillus inert （13.2%） in postmenopausal women, while Lactobaeillus inert （80.8%）, Lactobacillus crispatus （70.5 %）, Laetobacillus gasseri （67.6%） and Lactobacillus jensenii （39.7%） in reproductive age women. The content of 16s rDNA from vaginal lactobaeillus was more in reproductive-age women than in postmenopausal women [（87.3 ±0.8） ng/g total DNA vs. （35.1±3.6） ng / g total DNA, t=6. 514, P=0. 048]. Conclusions Lactobacillus strains colonizing in vaginal are different between postrnenopausal women and reproductive-age women, and the content of the vaginal lactobacillus is reduced significantly in postmenopausal women.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2014年第12期1334-1337,共4页
Chinese Journal of Geriatrics
关键词
阴道病
细菌性
乳杆菌属
聚合酶链反应
Vaginosis, bacterial
Lactobacillusl Polymerase chain reaction
