摘要
为建立一种简单快速、高效制备人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1的方法。利用大孔吸附树脂和硅胶柱层析对样品进行预处理,通过反相高效制备液相色谱,从红参的甲醇提取物中快速分离得到目标产物人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1,经检测,4种化合物的纯度均>98%。色谱柱为Polaris C18(250 mm×4.6 mm,10μm),以V(乙腈)∶V(水)=46∶54为流动相,恒梯度洗脱,流速4.0 mL/min,柱温室温,检测波长为203 nm,在此色谱条件下,它们得率分别为0.012%、0.013%、0.012%、0.013%。该方法操作简便,可重复进样,适用于制备高纯度稀有人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1。
A simple, fast and efficient method for the preparation of ginsenosides 20（ S）？Rg3, 20 （ R）？Rg3 , Rg5 and Rk1 was constructed. Using macroporous absorption resin and silica gel column chromatography, the samples were pretreated. Through RP？HPLC, target components 20（S）？Rg3, 20（ R）？Rg3 , Rg5 and Rk1 were rapidly separated from the methanol extracts of red ginseng with the purity over 98%. The separation was performed on a Polaris C18 （250 mm × 4？6 mm, 10 μm） at the room temperature by mobile phase of CH3CN ∶ H2O （46 ∶ 54） at a flow rate of 4？0 mL/min and the detection wavelength was 203 nm. Under these conditions, the yield rates of four ginsenosides were 0？012%, 0？013%, 0？012% and 0？013%, respectively. This method is effective and reliable for the preparation of 20（ S）？Rg3 , 20（ R）？Rg3 ,Rg5 and Rk1 from red ginseng.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2014年第5期591-594,共4页
Journal of Jilin Agricultural University
基金
国家重大研究项目(2011ZX09401-305-10-02)
国家科技支撑计划项目(2011BAI03B01)
