摘要
对采油微生物Acinetobacter junii.5-6摇瓶发酵培养基的碳源、起始pH进行优化,最后在最适培养基基础上分别添加微量元素(g/L)K2HPO41、NaH2PO41、FeSO4·7H2O 1、NaMoO41、MgSO4·7H2O 1、CaCl21,添加K2HPO4的效果最好;但是对菌浓的提高并不明显,且成本较高,所以不考虑在后续发酵添加微量元素。最终确定其最佳起始pH为5.5,最适培养基成分为:葵花籽油2%(v/v),糖蜜0.2%(m/v),NaCl 5 g/L,NaNO310 g/L,牛肉膏5 g/L。
The purpose of the research was to optinize the carbon source,initial pH of the nicroorganism Acinetobacterjunii.5-6 fermentation medium.At last these trace elements including K2HPO4,NaH2PO4,FeSO4 · 7H2O,NaMoO4,MgSO4 · 7H2O and CaCl2 were respectively added into the most suitable medium,each kind of trace elements was 1 g/L,the best was K2HPO4.However,the concentration of bacteria was not significantly improved,and the higher cost.So trace elements were not considered to add in the subsequent fermentation.Ultimately the optimal initial pH determined was 5.5.Optimal medium composition was Sunflower oil 2％ （v/v）,molasses 0.2％ （m/v）,NaC1 5 g/L,NaNO3 10 g/L and beef extract 5 g/L.
出处
《科学技术与工程》
北大核心
2014年第29期121-124,129,共5页
Science Technology and Engineering
基金
"863计划"资源环境技术领域项目(SS2013AA060902)资助
