摘要
Objective: To explore the mechanism of herb-partitioned moxibustion for ulcerative colitis(UC) through observing the colonic mucosal histopathological changes and the expression of nuclear factor kappaB(NF-kB) and peroxisome proliferators-activated receptor γ(PPARγ) mRNA of UC rats. Methods: Male SD rats were randomly divided into a normal group and a model group. UC model was established by general immunological plus local irritation method. After model identification, rats in the model group were randomly divided into a model group, a herb-partitioned moxibustion(HPM) group and a Western medicine(Salicylazosulfapyridine, SASP) group. Rats in the HPM group received treatment at bilateral Tianshu(ST 25) and Dachangshu(BL 25), two cones for each point, once a day for 7 d. SASP group rats were gavaged with SASP. The pathological scores were evaluated according to hematoxylin-eosin(HE) staining of colonic tissues. We used light microscopy to observe degree of colonic mucosal damage and the quantitative polymerase chain reaction(QPCR) to detect the expression of NF-κBp65 and PPARγ in colorectal mucosa. Results: Compared with the normal group, histopathological scores were significantly higher in the model group(P<0.05); compared with the model group, the scores were decreased significantly in the HPM group and the SASP group. Compared with the normal group, NF-κBp65 mRNA expression was increased with statistical significance in the model group(P<0.05); compared with the model group, NF-κBp65 mRNA expressions were decreased significantly in the HPM group and the SASP group. Compared with the normal group, PPARγ mRNA expression was increased significantly in the model group(P<0.05); compared with the model group, PPARγ mRNA expressions were decreased significantly in the HPM group and the SASP group. Conclusion: HPM could improve the mucosa damage of UC rats, which is possibly through down-regulating NF-κBp65 to achieve anti-inflammatory effect. Whether decreasing the PPARγ mRNA is possibly involved in preventing precancerosis will need further study.
Objective： To explore the mechanism of herb-partitioned moxibustion for ulcerative colitis（UC） through observing the colonic mucosal histopathological changes and the expression of nuclear factor kappaB（NF-kB） and peroxisome proliferators-activated receptor γ（PPARγ） mRNA of UC rats. Methods： Male SD rats were randomly divided into a normal group and a model group. UC model was established by general immunological plus local irritation method. After model identification, rats in the model group were randomly divided into a model group, a herb-partitioned moxibustion（HPM） group and a Western medicine（Salicylazosulfapyridine, SASP） group. Rats in the HPM group received treatment at bilateral Tianshu（ST 25） and Dachangshu（BL 25）, two cones for each point, once a day for 7 d. SASP group rats were gavaged with SASP. The pathological scores were evaluated according to hematoxylin-eosin（HE） staining of colonic tissues. We used light microscopy to observe degree of colonic mucosal damage and the quantitative polymerase chain reaction（QPCR） to detect the expression of NF-κBp65 and PPARγ in colorectal mucosa. Results： Compared with the normal group, histopathological scores were significantly higher in the model group（P〈0.05）; compared with the model group, the scores were decreased significantly in the HPM group and the SASP group. Compared with the normal group, NF-κBp65 mRNA expression was increased with statistical significance in the model group（P〈0.05）; compared with the model group, NF-κBp65 mRNA expressions were decreased significantly in the HPM group and the SASP group. Compared with the normal group, PPARγ mRNA expression was increased significantly in the model group（P〈0.05）; compared with the model group, PPARγ mRNA expressions were decreased significantly in the HPM group and the SASP group. Conclusion： HPM could improve the mucosa damage of UC rats, which is possibly through down-regulating NF-κBp65 to achieve anti-inflammatory effect. Whether decreasing the PPARγ mRNA is possibly involved in preventing precancerosis will need further study.
基金
supported by National Natural Science Foundation of China(No.81173331)
Scientific Research Budget Project of Shanghai Municipal Education Commission(No.2011JW46)
Science and Education Department Project for Youth,Shanghai Municipality Health Bureau(No.20124Y004)
