摘要
本文利用16S rDNA序列并结合形态学特征,鉴定实验室前期从油污土壤中分离的产表面活性剂菌株1098-3为大肠埃希氏菌(Escherichia coli)。通过单因子实验初步确定了其产生表面活性剂的最适条件:37℃,初始pH 7,0,转速200 r/min,培养基配比为可溶性淀粉2.5%,胰蛋白胨1.5%,氯化钠0.3%,磷酸二氢钾0.5%,氯化钙0.004%,硫酸铵0.6%,硫酸镁0.07%,酵母粉0.06%,500 mL三角瓶装液量为200 mL。在此条件下,发酵液表面张力由69.3 mN/m降至34.3mN/m,此时发酵液中表面活性剂相对浓度(RBC)为500。
A biosurfactant producing bacterium, which was isolated from crude oil pollution soil, was identified as Esche- richia coli by analyzing its morphological characteristics and 16S rDNA sequences. Single-factor experiments by using this strain for producing biosurfactant were conducted. Optimum culture conditions obtained for bacteria growth and biosurfactant production were as follows ： 37℃, initial pH 7.0, rotation rate of 200 r/min ; soluble starch 2.5%, tryptone 1.5%, NaCl 0.3% , KH2PO4 0.5% , CaCl2 0. 004% , （ NH4 ） 2 SO4 0.6% , MgSO4 0.07% , yeast powder 0.06% , 500 mL flask containing 200 mL medium. Under the optimized fermentation conditions, the surface tension of fermentation broth decreased from 69.3 mN/m to 34.3 mN/m and the relative biosurfactant concentration of fermentation broth was 500.
出处
《工业微生物》
CAS
CSCD
2014年第6期21-27,共7页
Industrial Microbiology
基金
中央高校基本科研业务费专项资金资助
关键词
生物表面活性剂
菌株鉴定
大肠杆菌
发酵条件优化
biosurfactant
strain identification
Escherichia coli
optimization of fermentation conditions
